Zeng Xuejun, Jia Hang, Zhang Xiao, Wang Xin, Wang Zhouli, Gao Zhenpeng, Yuan Yahong, Yue Tianli
College of Food Science and Engineering, Northwest A & F University, Yangling, 712100, China.
Laboratory of Quality & Safety Risk Assessment for Agri-products (Yangling), Ministry of Agriculture, Yangling, 712100, China.
Food Funct. 2021 Nov 15;12(22):11641-11655. doi: 10.1039/d1fo01729b.
The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model. Mice in the treatment group were orally administered with milk or kefir. The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing. Furthermore, the biomarkers associated with the gut barrier, inflammation, and cell proliferation regulators were evaluated. The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased. Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier. The / ratio and / ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (, and ) were decreased after supplementation of kefir. Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
本研究旨在利用氧化偶氮甲烷/葡聚糖硫酸钠（AOM/DSS）诱导的结直肠癌小鼠模型，研究开菲尔对调节结肠微生物群结构的结直肠癌（CRC）的疗效。治疗组小鼠口服牛奶或开菲尔。通过内转录间隔区2（ITS2）和16S rRNA高通量测序评估肠道微生物群组成。此外，还评估了与肠道屏障、炎症和细胞增殖调节因子相关的生物标志物。结果表明，肿瘤的大小和数量减少，免疫调节因子（TNF-α、IL-6和IL-17a）和肿瘤细胞增殖指标（Ki67、NF-κB和β-连环蛋白）均降低。短链脂肪酸（SCFAs）增加降低了结肠pH值，并有助于增强肠道屏障。在门水平上，/比率和/比率降低；补充开菲尔后，益生菌的相对丰度增加，病原菌（、和）减少。因此，开菲尔可以调节肠道微生物群组成，并改善AOM/DSS诱导的结直肠癌。
