Wang Sheng-Yao, Yen Wei-Chen, Chen Yen-Po, Shiu Jia-Shian, Chen Ming-Ju
Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
Nutrients. 2025 Jul 27;17(15):2447. doi: 10.3390/nu17152447.
HL1, isolated from kefir, exhibits antioxidant and anti-aging activities, defined here as improved cognitive function and reductions in oxidative stress and inflammatory markers. However, its poor milk viability limits application. This study developed a novel fermented milk by co-culturing HL1 with subsp. APL015 (APL15) to enhance fermentation and health benefits. HL1 and APL15 were co-cultured to produce fermented milk (FM), and fermentation performance, microbial viability, texture, and syneresis were evaluated. A D-galactose-induced aging BALB/c mouse model was used to assess cognitive function, oxidative stress, inflammation, antioxidant enzyme activity, and gut microbiota after 8 weeks of oral administration. FM reached pH 4.6 within 16 h, with high viable counts (~10 CFU/mL) for both strains. HL1 viability and texture were maintained, with smooth consistency and low syneresis. In vivo, FM improved cognitive behavior (Y-maze, Morris water maze), reduced oxidative damage (MDA), lowered IL-1β and TNF-α, and enhanced brain SOD levels. FM-fed mice exhibited increased short-chain fatty acid producers, higher cecal butyrate, and reduced . The co-cultured fermented milk effectively delivers HL1 and provides antioxidant, anti-inflammatory, and anti-aging effects in vivo, likely via gut-brain axis modulation. It shows promise as a functional food for healthy aging.
从开菲尔中分离出的HL1具有抗氧化和抗衰老活性，在此定义为改善认知功能以及降低氧化应激和炎症标志物水平。然而，其在牛奶中的存活率较低限制了其应用。本研究通过将HL1与亚种APL015（APL15）共培养来开发一种新型发酵乳，以增强发酵效果和健康益处。将HL1和APL15共培养以生产发酵乳（FM），并评估其发酵性能、微生物存活率、质地和脱水收缩。使用D-半乳糖诱导的衰老BALB/c小鼠模型在口服给药8周后评估认知功能、氧化应激、炎症、抗氧化酶活性和肠道微生物群。FM在16小时内达到pH 4.6，两种菌株的活菌数都很高（约10 CFU/mL）。HL1的存活率和质地得以维持，质地均匀且脱水收缩率低。在体内，FM改善了认知行为（Y迷宫、莫里斯水迷宫），减少了氧化损伤（丙二醛），降低了IL-1β和TNF-α水平，并提高了大脑中超氧化物歧化酶的水平。喂食FM的小鼠短链脂肪酸产生菌增加，盲肠丁酸盐含量更高，且……减少。共培养的发酵乳有效地传递了HL1，并在体内提供抗氧化、抗炎和抗衰老作用，可能是通过调节肠-脑轴实现的。它有望成为一种促进健康衰老的功能性食品。
