Ffrench M, Manel A M, Magaud J P, Fiere D, Adeleine P, Guyotat D, Bryon P A
Br J Haematol. 1987 Apr;65(4):419-23. doi: 10.1111/j.1365-2141.1987.tb04143.x.
Flow cytometry with propidium iodide and fluorescein isothiocyanate was used to study 46 cases of adult acute lymphoid leukaemia (ALL) before any form of chemotherapy. Cell proliferation was related to the other clinical and biological characteristics and its prognostic significance was evaluated. The following cell-cycle variables were determined: S, G2 + M, and the Low Protein Content fraction of G1 (LPC fraction). The L3 group, corresponding to B-ALL, had significantly higher proliferation than L1 and L2 (P less than 0.01). The proliferation rate was not significantly higher for T-ALL than for the other phenotypes. Complete remission was successfully induced significantly more often in cases with the LPC fraction under 50% (P less than 0.05). Failure was mainly related to resistance to chemotherapy. Of the four patients who died during aplasia, three had an LPC fraction below 25%. Duration of complete remission and survival were significantly shorter for L3 which is the most proliferative ALL (P less than 0.01). Survival was also found to be longer (P less than 0.05) when G2 + M was between 3.8% and 5.1%. This finding and the negative correlation between S and G2 + M (P less than 0.01) are discussed.
采用碘化丙啶和异硫氰酸荧光素进行流式细胞术，对46例成年急性淋巴细胞白血病（ALL）患者在接受任何形式化疗之前进行研究。细胞增殖与其他临床和生物学特征相关，并对其预后意义进行评估。测定了以下细胞周期变量：S期、G2+M期以及G1期低蛋白含量部分（LPC部分）。对应B-ALL的L3组增殖明显高于L1和L2组（P<0.01）。T-ALL的增殖率与其他表型相比无显著升高。LPC部分低于50%的患者完全缓解诱导成功率显著更高（P<0.05）。治疗失败主要与化疗耐药有关。在再生障碍期死亡的4例患者中，3例LPC部分低于25%。增殖性最强的ALL即L3组，其完全缓解持续时间和生存期显著更短（P<0.01）。当G2+M在3.8%至5.1%之间时，生存期也更长（P<0.05）。对这一发现以及S期与G2+M期之间的负相关关系（P<0.01）进行了讨论。
