College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
School of Pharmacy, Guangxi Medical University, Nanning 530021, China.
Anal Chem. 2021 Nov 16;93(45):15209-15215. doi: 10.1021/acs.analchem.1c03963. Epub 2021 Nov 2.
For better investigating the complicated relationships between HS and SO, simultaneously detecting and visualizing them with good selectivity is crucial. However, most sensing mechanisms for HS and SO probes are based on the addition reactions with the double bonds, which have no selectivity. In this work, by introducing an active triple bond into 4-dicyanovinyl-7-diethylamino-coumarin, we construct two unique sensors for not only distinguishing between HS and SO but also sensing HS and SO in a dual-ratiometric manner. Moreover, the modified sensor was successfully applied in living cells and zebrafish for discriminating HS and SO.
为了更好地研究 HS 和 SO 之间复杂的关系，同时具有良好的选择性来检测和可视化它们至关重要。然而，HS 和 SO 探针的大多数传感机制都是基于与双键的加成反应，因此没有选择性。在这项工作中，通过在 4-二氰基乙烯基-7-二乙氨基香豆素中引入一个活性三键，我们构建了两个独特的传感器，不仅可以区分 HS 和 SO，还可以以双重比率的方式感应 HS 和 SO。此外，该改良传感器成功应用于活细胞和斑马鱼中，用于区分 HS 和 SO。
