A near infrared fluorescence probe with dual-site for hydrogen sulfide and sulfur dioxide detection.

Both hydrogen sulfide (HS) and sulfur dioxide (SO) are regarded as double-edged swords. They are toxic gases at high concentration, and at low concentration they are beneficial to the human. Therefore, it is of great significance to develop single chemosensor which enable to detect them with different fluorescence signal changes. In this work, a novel dual-site fluorescence probe (AMN-SSPy) with near infrared emission (675 nm) was designed, which realized quantitative detection for HS and SO by fluorescence enhancement and fluorescence quenching, respectively. AMN-SSPy showed advantages such as excellent selectivity to HS and SO, strong anti-interference ability, high sensitivity for HS (LOD 1.03 µM for HS and 77.08 µM for SO) and low toxicity. In addition, AMN-SSPy possessed the capacity to successfully image the endogenous and exogenous HS, and it was also used to demonstrate that Ca could induce accumulation of HS in cell and zebrafish. Finally, the rapid detection of SO by AMN-SSPy in real samples was also established.