Li Haoyang, Liu Ying, Wang Yuhang, Li Jinshan, Li Yang, Zhang Guomei, Zhang Caihong, Shuang Shaomin, Dong Chuan
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Chumin College, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15;305:123523. doi: 10.1016/j.saa.2023.123523. Epub 2023 Oct 13.
Both hydrogen sulfide (HS) and sulfur dioxide (SO) are regarded as double-edged swords. They are toxic gases at high concentration, and at low concentration they are beneficial to the human. Therefore, it is of great significance to develop single chemosensor which enable to detect them with different fluorescence signal changes. In this work, a novel dual-site fluorescence probe (AMN-SSPy) with near infrared emission (675 nm) was designed, which realized quantitative detection for HS and SO by fluorescence enhancement and fluorescence quenching, respectively. AMN-SSPy showed advantages such as excellent selectivity to HS and SO, strong anti-interference ability, high sensitivity for HS (LOD 1.03 µM for HS and 77.08 µM for SO) and low toxicity. In addition, AMN-SSPy possessed the capacity to successfully image the endogenous and exogenous HS, and it was also used to demonstrate that Ca could induce accumulation of HS in cell and zebrafish. Finally, the rapid detection of SO by AMN-SSPy in real samples was also established.
硫化氢（HS）和二氧化硫（SO）都被视为双刃剑。它们在高浓度时是有毒气体，而在低浓度时对人体有益。因此，开发能够通过不同荧光信号变化检测它们的单一化学传感器具有重要意义。在这项工作中，设计了一种具有近红外发射（675 nm）的新型双位点荧光探针（AMN-SSPy），它分别通过荧光增强和荧光猝灭实现了对HS和SO的定量检测。AMN-SSPy表现出对HS和SO具有优异的选择性、强大的抗干扰能力、对HS的高灵敏度（HS的检测限为1.03 μM，SO的检测限为77.08 μM）以及低毒性等优点。此外，AMN-SSPy具有成功对内源性和外源性HS进行成像的能力，并且还被用于证明Ca可诱导细胞和斑马鱼中HS的积累。最后，还建立了AMN-SSPy对实际样品中SO的快速检测方法。
