Han Jinliang, Yang Sheng, Wang Benhua, Song Xiangzhi
College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, Hunan 410083, China.
Anal Chem. 2021 Mar 30;93(12):5194-5200. doi: 10.1021/acs.analchem.0c05266. Epub 2021 Mar 19.
Benzopyrylium-coumarin fluorescent probes for sensing hypochlorous acid (HClO) or sulfur dioxide (SO) are unable to distinguish between HClO and SO because the two compounds can react with the 4-position of benzopyrylium-coumarin dyes through the nucleophilic attack. In the current work, we introduced a phenoxazine moiety to the benzopyrylium-coumarin dye to synthesize a new fluorescent probe , which can dually sense HClO and SO and generate distinct fluorescence signals with rapid response time and high sensitivity and selectivity. Moreover, probe was also successfully utilized to detect intracellular HClO and SO in HeLa cells and zebrafish.
用于检测次氯酸（HClO）或二氧化硫（SO）的苯并吡喃鎓 - 香豆素荧光探针无法区分HClO和SO，因为这两种化合物可通过亲核攻击与苯并吡喃鎓 - 香豆素染料的4位发生反应。在当前工作中，我们将吩恶嗪部分引入苯并吡喃鎓 - 香豆素染料中以合成一种新的荧光探针，该探针可以双重检测HClO和SO，并以快速的响应时间、高灵敏度和高选择性产生不同的荧光信号。此外，探针还成功用于检测HeLa细胞和斑马鱼中的细胞内HClO和SO。
