National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, P. R. China.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, P. R. China.
J Antimicrob Chemother. 2022 Feb 2;77(2):351-355. doi: 10.1093/jac/dkab382.
To determine the transmission and molecular characteristics of blaNDM-producing Escherichia coli between companion animals and their healthcare providers at veterinary clinics in Guangzhou, China.
A total of 359 samples from companion animals and their healthcare providers were collected at 14 veterinary clinics in Guangzhou, China. Genomic characteristics and clonal relationships for blaNDM-positive E. coli and complete plasmid sequences were characterized based on WGS data from combined Illumina and MinION platform reads.
Forty-five blaNDM-positive bacteria were recovered from companion animals (n = 43) and their healthcare providers (n = 2) at 10 veterinary clinics. Overall, E. coli (73.3%, 33/45) and Klebsiella pneumoniae (13.3%, 6/45) were the most prevalent species among the seven species of blaNDM-positive bacteria. Four blaNDM variants (blaNDM-1, blaNDM-4, blaNDM-5 and blaNDM-7) were identified in 45 blaNDM-positive bacteria and blaNDM-5 was the most prevalent (77.8%, 35/45). WGS indicated that the most prevalent STs were ST405 (8/33), ST453 (6/33), ST457 (6/33) and ST410 (5/33) among the 33 blaNDM-positive E. coli isolates. Phylogenomics and PFGE analysis revealed that clonal spread of blaNDM-positive ST453 E. coli isolates between companion animals and their healthcare providers was evident. In addition, two novel IncFIB plasmids carrying blaNDM-4 (pF765_FIB and pG908_FIB) were found in this study and indicated that IS26 may promote the horizontal transmission of blaNDM between different plasmid types.
In this study we conducted a large-scale investigation on the prevalence of blaNDM-positive E. coli isolates from companion animals and their healthcare providers and revealed the clonal spread of blaNDM-positive E. coli isolates between these two groups.
研究中国广州兽医诊所的宠物及其医护人员携带的产 blaNDM 大肠杆菌的传播和分子特征。
从中国广州的 14 家兽医诊所共采集了 359 份宠物及其医护人员的样本。根据 Illumina 和 MinION 平台组合读数的 WGS 数据，对 blaNDM 阳性大肠杆菌的基因组特征和克隆关系以及完整质粒序列进行了表征。
从 10 家兽医诊所的 43 只宠物（n=43）和 2 名医护人员（n=2）中共分离出 45 株 blaNDM 阳性菌。在七种 blaNDM 阳性菌中，大肠杆菌（73.3%，33/45）和肺炎克雷伯菌（13.3%，6/45）最为常见。45 株 blaNDM 阳性菌中发现了 4 种 blaNDM 变体（blaNDM-1、blaNDM-4、blaNDM-5 和 blaNDM-7），其中 blaNDM-5 最为常见（77.8%，35/45）。WGS 表明，33 株 blaNDM 阳性大肠杆菌中最常见的 ST 型是 ST405（8/33）、ST453（6/33）、ST457（6/33）和 ST410（5/33）。系统发育基因组学和 PFGE 分析表明，blaNDM 阳性 ST453 大肠杆菌在宠物及其医护人员之间存在明显的克隆传播。此外，本研究还发现了两种携带 blaNDM-4 的新型 IncFIB 质粒（pF765_FIB 和 pG908_FIB），表明 IS26 可能促进了 blaNDM 基因在不同质粒类型之间的水平传播。
本研究对来自宠物及其医护人员的 blaNDM 阳性大肠杆菌分离株的流行情况进行了大规模调查，揭示了 blaNDM 阳性大肠杆菌分离株在这两组人群之间的克隆传播。
