Deng Yan, Hu Qian, Tang Bincheng, Ouyang Qingyuan, Hu Shenqiang, Hu Bo, Hu Jiwei, He Hua, Chen Guohong, Wang Jiwen
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225000, Jiangsu, China.
Anim Biosci. 2022 Jun;35(5):639-647. doi: 10.5713/ab.21.0382. Epub 2021 Oct 29.
This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese.
The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out.
An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes.
These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese.
本研究旨在克隆并比较四川白鹅和朗德鹅之间脱碘酶2（DIO2）基因的分子特征，并分析天府肉鹅中DIO2基因多态性与头部尺寸之间的关联。
通过聚合酶链反应和载体连接克隆DIO2基因的编码序列，并用DNAMAN软件进行比对。总共350只天府肉鹅用于对DIO2基因的多态性进行基因分型并测量头部尺寸。对DIO2基因多态性与头部尺寸之间进行关联分析。
获得了840bp的DIO2基因编码序列，比较分析确定了四川白鹅和朗德鹅之间的四个多态位点。进一步分析表明，这四个多态位点的优势等位基因分别为G、G、A和T，在天府肉鹅中杂合基因型的频率高于纯合基因型。与天府肉鹅非瘤鹅群体相比，瘤鹅群体的头部尺寸除鼻孔高度外均显著更高。然而，在非瘤鹅中，喙宽1、喙宽2、鼻孔长度、颅宽1和上颌长度在不同基因型或单倍型/双倍型之间存在显著差异。
这些结果表明，DIO2基因的多态性可被视为在非瘤鹅群体中选择头部较大的鹅的分子标记。
