Tung Bui Thanh, Thu Dang Kim, Thu Nguyen Thi Kim, Hai Nguyen Thanh
J Complement Integr Med. 2017 May 4;14(4):/j/jcim.2017.14.issue-4/jcim-2016-0116/jcim-2016-0116.xml. doi: 10.1515/jcim-2016-0116.
Background Zingiber officinale Roscoe has been used in traditional medicine for the treatment of neurological disorder. This study aimed to investigate the phenolic contents, antioxidant, acetylcholinesterase enzyme (AChE) inhibitory activities of different fraction of Z. officinale root grown in Vietnam. Methods The roots of Z. officinale are extracted with ethanol 96 % and fractionated with n-hexane, ethyl acetate (EtOAc) and butanol (BuOH) solvents. These fractions evaluated the antioxidant activity by 1,1-Diphenyl -2-picrylhydrazyl (DPPH) assay and AChE inhibitory activity by Ellman's colorimetric method. Results Our data showed that the total phenolic content of EtOAc fraction was highest equivalents to 35.2±1.4 mg quercetin/g of fraction. Our data also demonstrated that EtOAc fraction had the strongest antioxidant activity with IC50 was 8.89±1.37 µg/mL and AChE inhibitory activity with an IC50 value of 22.85±2.37 μg/mL in a dose-dependent manner, followed by BuOH fraction and the n-hexane fraction is the weakest. Detailed kinetic analysis indicated that EtOAc fraction was mixed inhibition type with Ki (representing the affinity of the enzyme and inhibitor) was 30.61±1.43 µg/mL. Conclusions Our results suggest that the EtOAc fraction of Z. officinale may be a promising source of AChE inhibitors for Alzheimer's disease.
背景 生姜已被用于传统医学中治疗神经系统疾病。本研究旨在调查越南种植的生姜根不同部位的酚类成分、抗氧化、乙酰胆碱酯酶（AChE）抑制活性。方法 用96%乙醇提取生姜根，并用正己烷、乙酸乙酯（EtOAc）和丁醇（BuOH）溶剂进行分离。这些部位通过1,1-二苯基-2-苦基肼（DPPH）法评估抗氧化活性，通过埃尔曼比色法评估AChE抑制活性。结果 我们的数据表明，乙酸乙酯部位的总酚含量最高，相当于35.2±1.4毫克槲皮素/克部位。我们的数据还表明，乙酸乙酯部位具有最强的抗氧化活性，IC50为8.89±1.37微克/毫升，且对AChE的抑制活性呈剂量依赖性，IC50值为22.85±2.37微克/毫升，其次是丁醇部位，正己烷部位最弱。详细的动力学分析表明，乙酸乙酯部位为混合抑制类型，Ki（代表酶与抑制剂的亲和力）为30.61±1.43微克/毫升。结论 我们的结果表明，生姜的乙酸乙酯部位可能是治疗阿尔茨海默病的AChE抑制剂的一个有前景的来源。
