Departments of Medicine, Genetics and Development, Urology, and Systems Biology, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, NY, 10032, USA.
Departments of Medicine, Genetics and Development, Urology, and Systems Biology, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, NY, 10032, USA.
Cancer Lett. 2022 Jan 28;525:108-114. doi: 10.1016/j.canlet.2021.10.035. Epub 2021 Oct 30.
The recent advent of single-cell RNA-sequencing technology has provided new fundamental insights into the heterogeneity of the prostate epithelium. Several independent studies have described extensive heterogeneity of the luminal epithelial compartment, including a major division between a novel population of luminal cells located in the proximal region of the prostate ducts versus luminal cells located more distally. Proximal luminal cells as well as novel periurethral cells display increased progenitor potential in organoid culture and tissue reconstitution assays, but not in lineage-tracing analyses during prostate homeostasis, suggesting context-dependent plasticity of these populations. Here we describe and synthesize recent findings regarding the epithelial cell populations in the mouse prostate, draw comparisons to the human prostate, and address the relevance of these findings to prostate diseases and cancer.
单细胞 RNA 测序技术的最新出现为前列腺上皮细胞的异质性提供了新的基本见解。几项独立的研究描述了腔上皮细胞区室的广泛异质性，包括位于前列腺导管近端的新型腔细胞与位于更远端的腔细胞之间的主要划分。在类器官培养和组织重建分析中，近端腔细胞和新型尿道周围细胞显示出增加的祖细胞潜力，但在前列腺稳态的谱系追踪分析中则没有，这表明这些细胞群体的上下文依赖性可塑性。在这里，我们描述并综合了关于小鼠前列腺中上皮细胞群体的最新发现，与人类前列腺进行了比较，并探讨了这些发现与前列腺疾病和癌症的相关性。
