Pen-Tung Sah Institute of Micro-Nano Science and Technology, College of Chemistry and Chemical Engineering, School of Electronic Science and Engineering (National Model Microelectronics College), Xiamen University, Xiamen 361005, China.
Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo 315000, China.
Anal Chem. 2021 Nov 16;93(45):15033-15041. doi: 10.1021/acs.analchem.1c02758. Epub 2021 Nov 3.
Rapid and automated detection of acute myocardial infarction (AMI) at its developing stage is very important due to its high mortality rate. To quantitatively diagnose AMI, Myo, CK-MB, and cTnI are chosen as three biomarkers, which are usually detected through an immunosorbent assay, such as the enzyme-linked immunosorbent assay. However, the approach poses many drawbacks, such as long detection time, the cumbersome process, the need for professionals, and the difficulty of realizing automatic operation. Here, a multichannel digital microfluidic (DMF) thermal control chip integrated with a sandwich-based immunoassay strategy is proposed for the automated, rapid, and sensitive detection of AMI biomarkers. A miniaturized temperature control module is integrated on the back of the DMF chip, meeting the temperature requirement for the immunoassay. With this DMF thermal control chip, sample and reagent consumption are reduced to several microliters, significantly alleviating reagent consumption and sample dependence, and the automated and multichannel detection of biomarkers can be achieved. In this work, the simultaneously noninvasive detection of the human serum sample containing the three biomarkers of AMI is also achieved within 30 min, which improves the diagnostic accuracy of AMI. Due to the features of automation and miniaturization, the multichannel immunosensor can be used in community hospitals to increase the speed of diagnosis of patients with various acute diseases.
由于急性心肌梗死（AMI）的死亡率很高，因此在其发展阶段快速、自动地检测它非常重要。为了定量诊断 AMI，选择肌红蛋白（Myo）、肌酸激酶同工酶（CK-MB）和心肌肌钙蛋白 I（cTnI）作为三种生物标志物，通常通过免疫吸附测定法（如酶联免疫吸附测定法）进行检测。然而，这种方法存在许多缺点，例如检测时间长、过程繁琐、需要专业人员以及难以实现自动化操作。在这里，提出了一种集成基于三明治免疫测定策略的多通道数字微流控（DMF）热控芯片，用于自动、快速和灵敏地检测 AMI 生物标志物。在 DMF 芯片的背面集成了一个小型化的温度控制模块，满足免疫测定的温度要求。使用这种 DMF 热控芯片，样品和试剂的消耗减少到几微升，显著减轻了试剂消耗和样品依赖性，并且可以实现生物标志物的自动化和多通道检测。在这项工作中，还在 30 分钟内实现了同时非侵入式检测含有 AMI 三种生物标志物的人血清样品，提高了 AMI 的诊断准确性。由于具有自动化和微型化的特点，多通道免疫传感器可用于社区医院，以加快各种急性疾病患者的诊断速度。
