Ji Jiajian, Hu Chenxuan, Pang Xinpei, Liang Jiancong, Huang Qi, Hu Siyi, Mei Qian, Ma Hanbin
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (CAS), Suzhou 215163, China.
ACS Omega. 2024 Feb 19;9(9):10937-10944. doi: 10.1021/acsomega.3c10312. eCollection 2024 Mar 5.
In this paper, a digital microfluidic thermal control system was introduced for the stable polymerase chain reaction (PCR). The system consists of a thermoelectric cooler unit, a thermal control board, and graphical-user-interface software capable of simultaneously achieving temperature control and on-chip droplet observation. A fuzzy proportional-integral-derivative (PID) method was developed for this system. The simulation analysis was performed to evaluate the temperature of different reagents within the chip. Based on the results, applying fuzzy PID control for PCR will enhance the thermal stability by 67.8% and save the time by 1195 s, demonstrating excellent dynamic response capability and thermal robustness. The experimental results are consistent with the simulation results on the planar temperature distribution, with a data consistency rate of over 99%. The PCR validation was carried out on this system, successfully amplifying the rat GAPDH gene at a concentration of 193 copies/μL. This work has the potential to be useful in numerous existing lab-on-a-chip applications.
本文介绍了一种用于稳定聚合酶链反应（PCR）的数字微流控热控制系统。该系统由一个热电冷却器单元、一个热控板和能够同时实现温度控制和芯片上液滴观察的图形用户界面软件组成。为此系统开发了一种模糊比例积分微分（PID）方法。进行了模拟分析以评估芯片内不同试剂的温度。基于结果，将模糊PID控制应用于PCR将使热稳定性提高67.8%，并节省1195秒时间，显示出出色的动态响应能力和热鲁棒性。实验结果与平面温度分布的模拟结果一致，数据一致性率超过99%。在该系统上进行了PCR验证，成功地以193拷贝/μL的浓度扩增了大鼠GAPDH基因。这项工作有可能在众多现有的芯片实验室应用中发挥作用。
