Department of Mortuary Science, College of Bio-convergence, Eulji University, 553, Sanseong-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13135, Korea.
Department of Parasitology, Dankook University College of Medicine, 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungcheongnam-do 31116, Korea.
J Parasitol. 2022 Jan 1;108(1):70-78. doi: 10.1645/21-49.
Although Clonorchis sinensis is a parasite that still infects many people in East Asia, its genetics remain largely unknown. We conducted ancient DNA analysis of C. sinensis eggs obtained from a Joseon period mummy newly discovered in South Korea. Clonorchis sinensis DNA was amplified for internal transcribed spacer 1, cytochrome c oxidase subunit 1, and NADH dehydrogenase subunit 2 and 5 genes. The results of BLAST/NCBI showed that the consensus sequences were 98.24 to 100% identical to the modern and ancient C. sinensis sequences reported from Korea, China, Japan, and other Asian countries. Our report helps to fill in the genetic profile of ancient C. sinensis strains that infected East Asian people hundreds of years ago.
尽管华支睾吸虫仍然在东亚的许多地区感染人群，但它的基因情况在很大程度上仍是未知的。我们对新发现于韩国的一具朝鲜时期木乃伊中的华支睾吸虫卵进行了古 DNA 分析。我们扩增了内部转录间隔区 1、细胞色素 c 氧化酶亚基 1、NADH 脱氢酶亚基 2 和 5 基因对华支睾吸虫进行分析。BLAST/NCBI 的结果显示，这些共识序列与来自韩国、中国、日本和其他亚洲国家的现代和古代华支睾吸虫序列完全一致，相似度为 98.24%到 100%。我们的报告有助于填补数百年前感染东亚人群的古代华支睾吸虫株的遗传特征。
