Department of Toxicology, Medical University of Gdansk, Gdansk, Poland.
Department of Clinical Nutrition, Medical University of Gdansk, Gdansk, Poland.
Int J Obes (Lond). 2022 Feb;46(2):408-416. doi: 10.1038/s41366-021-01013-y. Epub 2021 Nov 3.
BACKGROUND/OBJECTIVES: Oxylipins are polyunsaturated fatty acid derivatives involved in the regulation of various processes, including chronic inflammation, insulin resistance and hepatic steatosis. They can be synthesized in various tissues, including adipose tissue. There is some evidence that obesity is associated with the deregulation of serum oxylipin levels. The aim of this study was to evaluate the effect of bariatric surgery (one-anastomosis gastric bypass) on the serum levels of selected oxylipins and their fatty acid precursors and to verify the hypothesis that their changes after surgery can contribute to the resolution of inflammation. Moreover, we compared the oxylipin levels (prostaglandin E2, 13-HODE, maresin 1 and resolvin E1), fatty acids and the expression of enzymes that synthesize oxylipins in adipose tissue of lean controls and subjects with severe obesity.
SUBJECTS/METHODS: The study included 50 patients with severe obesity that underwent bariatric surgery and 41 subjects in lean, control group. Fatty acid content was analyzed by GC-MS, oxylipin concentrations were measured with immunoenzymatic assay kits and real-time PCR analysis was used to assess mRNA levels in adipose tissue.
Our results show increased expression of some enzymes that synthesize oxylipins in adipose tissue and alterations in the levels of oxylipins in both adipose tissue and serum of subjects with obesity. After bariatric surgery, the levels of anti-inflammatory oxylipins increased, whereas pro-inflammatory oxylipins decreased.
In patients with obesity, the metabolism of oxylipins is deregulated in adipose tissue, and their concentrations in serum are altered. Bariatric surgery modulates the serum levels of pro- and anti-inflammatory oxylipins, which may contribute to the resolution of inflammation.
背景/目的：氧化脂类是参与调节多种过程的多不饱和脂肪酸衍生物，包括慢性炎症、胰岛素抵抗和肝脂肪变性。它们可以在包括脂肪组织在内的各种组织中合成。有一些证据表明，肥胖与血清氧化脂类水平的失调有关。本研究旨在评估减肥手术（单吻合胃旁路术）对选定氧化脂类及其脂肪酸前体的血清水平的影响，并验证手术后它们的变化有助于炎症的消退的假设。此外，我们比较了瘦对照组和严重肥胖组脂肪组织中的氧化脂类水平（前列腺素 E2、13-HODE、maresin 1 和 resolvin E1）、脂肪酸和合成氧化脂类的酶的表达。
受试者/方法：该研究包括 50 名接受减肥手术的严重肥胖患者和 41 名瘦对照组受试者。通过 GC-MS 分析脂肪酸含量，用免疫酶试剂盒测量氧化脂类浓度，并用实时 PCR 分析评估脂肪组织中的 mRNA 水平。
我们的结果表明，肥胖受试者脂肪组织中合成氧化脂类的一些酶的表达增加，并且氧化脂类在脂肪组织和血清中的水平都发生了改变。减肥手术后，抗炎性氧化脂类的水平增加，而促炎性氧化脂类的水平降低。
在肥胖患者中，脂肪组织中氧化脂类的代谢失调，其血清浓度也发生改变。减肥手术调节了促炎和抗炎性氧化脂类的血清水平，这可能有助于炎症的消退。
