Zafari Jaber, Abbasinia Hossein, Gharehyazi Hediyeh, Javani Jouni Fatemeh, Jamali Saeed, Razzaghi Mohammadreza
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
J Lasers Med Sci. 2021 May 1;12:e17. doi: 10.34172/jlms.2021.17. eCollection 2021.
Cancer is one of the most important problems in the world. Low-level laser therapy (LLLT) has been emerged as a new approach, having both stimulation and inhibition effects on cellular function. The goal of this study was to analyze and compare the different concentrations of cisplatin and wavelengths of laser therapy on the LnCap cell lines. LnCap cells were cultured and treated with different concentrations of cisplatin (0.1, 0.4, 0.8, 1.2 and 2 µg/mL for 24 hours) and wavelengths of laser therapy (610, 630 and 810 nm) (0.45 J/cm) separately. The viability of cells was examined by MTT assay and IC50 was also calculated. Furthermore, a combination of cisplatin IC50 (24 hours) and different wavelengths of the laser was examined. The results of this study showed that 2 µg/mL of cisplatin has the most significant reduction effect on the cell viability of the LnCap cell line. Cisplatin decreased the viability of cells in a dose-dependent manner. Moreover, IC50 of cisplatin was 1.24 µg/mL. On the other hand, LLLT with wavelengths of 610, 630 and 810 nm did not show notable biological effects on cell viability. As known, cisplatin has the capability to reduce the viability of LnCap cell lines. However, LLLT cannot be a remarkable option for the treatment of prostate cancer. Therefore, although laser therapy showed praiseful therapeutic activity against some cancer cell lines, in this study the results indicated that defined laser wavelengths had no inhibitory effects against the prostate cancer cell line.
癌症是世界上最重要的问题之一。低强度激光疗法（LLLT）已成为一种新方法，对细胞功能具有刺激和抑制作用。本研究的目的是分析和比较不同浓度顺铂和不同波长激光疗法对LnCap细胞系的影响。将LnCap细胞培养后分别用不同浓度的顺铂（0.1、0.4、0.8、1.2和2µg/mL，处理24小时）和不同波长的激光疗法（610、630和810nm，能量密度为0.45J/cm²）进行处理。通过MTT法检测细胞活力并计算IC50。此外，还检测了顺铂IC50（24小时）与不同波长激光的联合作用。本研究结果表明，2µg/mL的顺铂对LnCap细胞系的细胞活力具有最显著的降低作用。顺铂以剂量依赖性方式降低细胞活力。此外，顺铂的IC50为1.24µg/mL。另一方面，波长为610、630和810nm的低强度激光疗法对细胞活力未显示出显著的生物学效应。众所周知，顺铂有降低LnCap细胞系活力的能力。然而，低强度激光疗法并非治疗前列腺癌的显著选择。因此，尽管激光疗法对某些癌细胞系显示出令人称赞的治疗活性，但在本研究中结果表明特定的激光波长对前列腺癌细胞系没有抑制作用。
