Sinaei Maryam, Sekhavat Saba, Zafari Jaber, Moradzadegan Atousa
Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran.
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2024 Jul 9;15:e22. doi: 10.34172/jlms.2024.22. eCollection 2024.
Breast cancer ranks among the most prevalent malignancies, and its prompt diagnosis significantly amplifies the prospects of successful treatment. Approximately one in seven women will experience a breast cancer diagnosis in their lifetime. Stromal cells and their secreted factors exert various effects on tumor growth, impacting proliferation, invasion, and metastasis. Research has emphasized the significant impact of proteins secreted by adipose tissue on breast cancer proliferation, surpassing the influence of factors released by other cell types. Yet, the specific transcription factors and cofactors involved in adipokine expression in the tumor microenvironment remain enigmatic. In this study, adipocyte cells were cultured and exposed to 980 nm and 650 nm Photobiomodulation. The MDA-MD-231 cells (triple negative cancer cell line) were cultured with a conditioned medium from laser-treated cells. The real-time assay was employed to analyze the gene expression level changes involved in apoptosis. Results showed that the irradiated conditioned medium at 980 nm and 650 nm caused a reduction in cell viability of cancer cells. Conversely, the conditioned medium from the irradiated cells triggered an increase in the expression of , , and genes, alongside a decrease in gene expression. The findings highlighted the potential of the laser-treated conditioned medium to induce apoptosis pathways in cancer cells, demonstrating a promising avenue for further research in utilizing low-level laser therapy in breast cancer treatment.
乳腺癌是最常见的恶性肿瘤之一，其早期诊断显著提高了成功治疗的前景。大约每七名女性中就有一人在一生中会被诊断出患有乳腺癌。基质细胞及其分泌的因子对肿瘤生长产生多种影响，影响增殖、侵袭和转移。研究强调了脂肪组织分泌的蛋白质对乳腺癌增殖的重大影响，超过了其他细胞类型释放的因子的影响。然而，肿瘤微环境中参与脂肪因子表达的特定转录因子和辅助因子仍然不明。在本研究中，培养脂肪细胞并使其暴露于980纳米和650纳米的光生物调节。将MDA-MD-231细胞（三阴性癌细胞系）与来自激光处理细胞的条件培养基一起培养。采用实时分析来分析参与细胞凋亡的基因表达水平变化。结果表明，980纳米和650纳米的照射条件培养基导致癌细胞的细胞活力降低。相反，来自照射细胞的条件培养基引发了、和基因表达的增加，同时基因表达降低。这些发现突出了激光处理的条件培养基在癌细胞中诱导凋亡途径的潜力，为在乳腺癌治疗中利用低水平激光疗法的进一步研究展示了一条有前景的途径。
