Mostafavinia Atarodsadat, Amini Abdollah, Ahmadi Houssein, Rezaei Fatemehalsadat, Ghoreishi Seyed Kamran, Chien Sufan, Bayat Mohammad
Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran.
J Lasers Med Sci. 2021 Jul 24;12:e40. doi: 10.34172/jlms.2021.40. eCollection 2021.
Herein, the individual and combined effects of photobiomodulation (PBM) and arginine (ARG) on the wound healing course of an experimental model of a slow healing wound (ulcer) in rats were assessed. A total of 108 male rats were divided into 6 groups: control; lower energy density (low)-PBM; arginine ointment (ARG); low-PBM+ARG; high energy density (high)-PBM; and high-PBM+ARG. In each rat, one ischemic wound in the center of a bipedicle flap and one non-ischemic wound out of the flap were created. Both wounds were treated in the experimental groups. Microbial growth, wound area, and wound strength were assessed on days 0, 5, 10, 15, and 20 after wound infliction. All non-ischemic wounds closed before day 15. High-PBM+ARG and ARG significantly increased wound closure rates compared to the control group (LSD test, = 0.000, and = 0.001, respectively) on day 10. All slow healing wounds were open on day 15 but closed completely before day 20. Low-PBM+ARG and high-PBM significantly increased wound strength (stress high load, SHL) on day 10 compared to the control group (LSD test, = 0.001, and = 0.000, respectively). ARG, high-PBM, and low-PBM+ARG significantly increased wound closure rates on day 15 relative to the control group (LSD test, = 0.000, = 0.000, and = 0.001, respectively). High-PBM and low-PBM+ARG have biostimulatory and antibacterial effects on slow-healing wounds, which were shown by significant increases in wound closure rates, wound strength, and inhibition of growth.
在此，评估了光生物调节（PBM）和精氨酸（ARG）对大鼠慢性愈合伤口（溃疡）实验模型伤口愈合过程的单独及联合作用。总共108只雄性大鼠被分为6组：对照组；低能量密度（低）-PBM组；精氨酸软膏（ARG）组；低-PBM+ARG组；高能量密度（高）-PBM组；以及高-PBM+ARG组。在每只大鼠身上，在双蒂皮瓣中心制作一个缺血性伤口，并在皮瓣外制作一个非缺血性伤口。实验组对两个伤口均进行处理。在伤口形成后的第0、5、10、15和20天评估微生物生长、伤口面积和伤口强度。所有非缺血性伤口在第15天前愈合。在第10天，与对照组相比，高-PBM+ARG组和ARG组显著提高了伤口闭合率（LSD检验，分别为=0.000和=0.001）。所有慢性愈合伤口在第15天仍未愈合，但在第20天前完全愈合。与对照组相比，在第10天，低-PBM+ARG组和高-PBM组显著提高了伤口强度（应力高负荷，SHL）（LSD检验，分别为=0.001和=0.000）。相对于对照组，在第15天，ARG组、高-PBM组和低-PBM+ARG组显著提高了伤口闭合率（LSD检验，分别为=0.000、=0.000和=0.001）。高-PBM组和低-PBM+ARG组对慢性愈合伤口具有生物刺激和抗菌作用，这表现为伤口闭合率、伤口强度显著增加以及细菌生长受到抑制。
