Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok, 10110, Thailand.
Department of Chemistry, Colorado State University, Fort Collins, CO, 80523-1872, United States.
Talanta. 2022 Jan 15;237:122983. doi: 10.1016/j.talanta.2021.122983. Epub 2021 Oct 20.
This work presents an all-in-one origami paper-based electrochemical platform for simple and inexpensive l-cysteine (Cys) detection using Cys as a monomer for modifying electrode surfaces. The proposed method combines the steps of electropolymerization and detection into a single device to offer a highly convenient method for the end-user. In comparison, the sensitivity toward Cys detection is a significantly increased using this modified electrode. The developed device provided a linear concentration range of 10-800 μM with a limit of detection of 5.5 μM. For application, the device was successfully applied to detect Cys in different food products such as wheat flour, bread, and cake with satisfactory results, yielding excellent intra-day and inter-day relative standard deviations (1.5-4.9%) and recoveries (84.2-110.8%). This discovery is important from the viewpoint of the development of Cys detection in other applications in the future.
这项工作提出了一个一体化折纸纸基电化学平台，用于使用半胱氨酸 (Cys) 作为修饰电极表面的单体进行简单且廉价的 L-半胱氨酸 (Cys) 检测。该方法将聚合和检测步骤结合到单个设备中，为最终用户提供了一种非常方便的方法。相比之下，使用这种修饰电极可以显著提高对半胱氨酸检测的灵敏度。该开发的设备提供了 10-800 μM 的线性浓度范围，检测限为 5.5 μM。在应用方面，该设备成功地应用于检测不同食品产品（如小麦粉、面包和蛋糕）中的半胱氨酸，结果令人满意，日内和日间相对标准偏差（1.5-4.9%）和回收率（84.2-110.8%）均非常出色。从未来在其他应用中开发半胱氨酸检测的角度来看，这一发现非常重要。
