Zhao Feifei, Yuan Zhili, Wen Weidong, Huang Zhongyu, Mao Xuewei, Zhou Mingguo, Hou Yiping
College of Plant Protection, Nanjing Agricultural University, Jiangsu, China.
Front Plant Sci. 2022 Oct 24;13:1011709. doi: 10.3389/fpls.2022.1011709. eCollection 2022.
is a destructive filamentous fungus, which widely exists in wheat and other cereal crops. Cysteine and Methionine are unique sulfur-containing amino acids that play an essential role in protein synthesis and cell life, but their functions and regulation in remain largely unknown. Here we identified two proteins, FgMet3 and FgMet14 in , which are related to the synthesis of cysteine and methionine. We found FgMet3 and FgMet14 were localized to the cytoplasm and there was an interaction between them. or deletion mutants (Δ and Δ) were deficient in vegetative growth, pigment formation, sexual development, penetrability and pathogenicity. With exogenous addition of cysteine and methionine, the vegetative growth and penetrability could be completely restored in Δ and Δ, while sexual reproduction could be fully restored in Δ and partially restored in Δ. Δ and Δ exhibited decreased sensitivity to Congo red stress and increased sensitivity to SDS, NaCl, KCl, Sorbitol, Menadione, and Zn ion stresses. Moreover, FgMet3 and FgMet14 nonspecifically regulate the sensitivity of to fungicides. In conclusion, FgMet3 and FgMet14 interacted to jointly regulate the development, pathogenicity, pigment formation, sensitivity to fungicides and stress factors in .
是一种具有破坏性的丝状真菌，广泛存在于小麦和其他谷类作物中。半胱氨酸和蛋氨酸是独特的含硫氨基酸，在蛋白质合成和细胞生命中发挥着重要作用，但它们在 中的功能和调控在很大程度上仍不清楚。在这里，我们在 中鉴定出两种与半胱氨酸和蛋氨酸合成相关的蛋白质，FgMet3和FgMet14。我们发现FgMet3和FgMet14定位于细胞质中，并且它们之间存在相互作用。 或 缺失突变体（Δ 和 Δ）在营养生长、色素形成、有性发育、穿透性和致病性方面存在缺陷。通过外源添加半胱氨酸和蛋氨酸，Δ 和 Δ 的营养生长和穿透性可以完全恢复，而有性生殖在 Δ 中可以完全恢复，在 Δ 中可以部分恢复。Δ 和 Δ 对刚果红胁迫的敏感性降低，对 SDS、NaCl、KCl、山梨醇、甲萘醌和锌离子胁迫的敏感性增加。此外，FgMet3和FgMet14非特异性地调节 对杀菌剂的敏感性。总之，FgMet3和FgMet14相互作用共同调节 中的发育、致病性、色素形成、对杀菌剂的敏感性和胁迫因子。
