Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
J Proteomics. 2022 Jan 16;251:104408. doi: 10.1016/j.jprot.2021.104408. Epub 2021 Nov 2.
Vibrio cholerae can cause pandemic cholera in humans. The bacterium resides in aquatic environments worldwide. Identification of risk factors of V. cholerae in aquatic products is imperative for assuming food safety. In this study, we determined virulence-associated genes, cross-resistance between antibiotics and heavy metals, and genome fingerprinting profiles of non O1/O139 V. cholerae isolates (n = 20) recovered from 16 species of consumable aquatic animals. Secretomes and proteomes of V. cholerae with distinct genotypes and phenotypes were obtained by using two-dimensional gel electrophoresis (2D-GE) and/or liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques. Comparative secretomic analysis revealed 4 common and 45 differential extracellular proteins among 20 V. cholerae strains, including 13 virulence- and 8 resistance-associated proteins. A total of 21,972 intracellular proteins were identified, and comparative proteomic analysis revealed 215 common and 913 differential intracellular proteins, including 22 virulence- and 8 resistance-associated proteins. Additionally, different secretomes and proteomes were observed between V. cholerae isolates of fish and shellfish origins. A number of novel proteins with unknown function and strain-specific proteins were also discovered in the V. cholerae isolates. SIGNIFICANCE: V. cholerae can cause pandemic cholera in humans. The bacterium is distributed in aquatic environments worldwide. Identification of risk factors of V. cholerae in aquatic products is imperative for assuming food safety. Non-O1/O139 V. cholerae has been reported to cause sporadic cholera-like diarrhea and bacteremia diseases, which indicates virulence factors rather than the major cholera toxin (CT) exist. This study for the first time investigated proteomes and secretomes of non-O1/O139 V. cholerae originating from aquatic animals. This resulted in the identification of a number of virulence and coresistance-related factors, as well as novel proteins and strain-specific proteins in V. cholerae isolates recovered from 16 species of consumable aquatic animals. These results fill gaps for better understanding of pathogenesis and resistance of V. cholerae, and also support the increasing need for novel diagnosis and vaccine targets against the leading waterborne pathogen worldwide.
霍乱弧菌可引起人类大流行霍乱。该细菌存在于全球的水生环境中。鉴定水产品中霍乱弧菌的危险因素对于确保食品安全至关重要。在本研究中，我们确定了非 O1/O139 霍乱弧菌分离株（n=20）的毒力相关基因、抗生素和重金属之间的交叉耐药性以及基因组指纹图谱，这些分离株来自 16 种可食用水生动物。通过二维凝胶电泳（2D-GE）和/或液相色谱-串联质谱（LC-MS/MS）技术获得具有不同基因型和表型的霍乱弧菌的分泌组和蛋白质组。比较分泌组分析显示，在 20 株霍乱弧菌菌株中存在 4 种共同和 45 种差异的细胞外蛋白，包括 13 种毒力相关和 8 种耐药相关蛋白。共鉴定出 21972 种细胞内蛋白，比较蛋白质组分析显示，20 株霍乱弧菌中存在 215 种共同和 913 种差异的细胞内蛋白，包括 22 种毒力相关和 8 种耐药相关蛋白。此外，还观察到来自鱼类和贝类来源的霍乱弧菌分离株之间的不同分泌组和蛋白质组。还在霍乱弧菌分离株中发现了许多具有未知功能的新型蛋白和菌株特异性蛋白。意义：霍乱弧菌可引起人类大流行霍乱。该细菌分布在全球的水生环境中。鉴定水产品中霍乱弧菌的危险因素对于确保食品安全至关重要。已报道非 O1/O139 霍乱弧菌可引起散发性霍乱样腹泻和菌血症疾病，这表明存在毒力因子而不是主要霍乱毒素（CT）。本研究首次调查了来自水生动物的非 O1/O139 霍乱弧菌的蛋白质组和分泌组。这导致鉴定出许多毒力和核心耐药相关因素，以及从 16 种可食用水生动物中回收的霍乱弧菌分离株中的新型蛋白和菌株特异性蛋白。这些结果填补了更好地了解霍乱弧菌发病机制和耐药性的空白，也支持了针对全球主要水源性病原体的新型诊断和疫苗靶标的日益增长的需求。
