Zhang Taofeng, Chen Yihuan, Lin Weixun, Zheng Jiehua, Liu Yiyuan, Zou Juan, Cai Jiehui, Chen Yaokun, Li Zhiyang, Chen Yexi
Department of General Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.
Department of Ultrasound in Obstetrics and Gynecology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.
Front Genet. 2021 Oct 19;12:710412. doi: 10.3389/fgene.2021.710412. eCollection 2021.
Thyroid cancer (THCA) is a common endocrine malignancy. With increasing incidence and low mortality, balancing the therapeutic approach is an inevitable issue. This study aimed to confirm the role of miR-222-3p and its target genes in THCA survival and immune infiltration. From different expression analyses based on the GEO and TCGA databases, we predicted and subsequently identified the key target genes of miR-222-3p. We then explored the expression, enrichment, pairwise correlation, protein expression, survival analysis, principal component analysis, and immune significance of the critical genes using bioinformatics analysis. The present study demonstrated that , , , , , , and are critical genes. The miR-222-3p was highly expressed, probably leading to low and high expression in THCA tissues. Low expression indicated favorable prognosis in THCA patients, and high expression indicated poor prognosis. Seven critical genes were significantly related to gender, age, race, tumor stage, and lymph node metastasis. In addition, the seven-gene biomarker exhibited a certain diagnostic value. Finally, expression was closely correlated with immune cells, especially B cells and CD4 T cells. The miR-222-3p and its critical target genes could be promising biomarkers for the prognosis of THCA and may emerge as key regulators of immune infiltration in THCA.
甲状腺癌（THCA）是一种常见的内分泌恶性肿瘤。随着发病率的上升和死亡率的降低，平衡治疗方法是一个不可避免的问题。本研究旨在证实miR-222-3p及其靶基因在THCA生存和免疫浸润中的作用。通过基于GEO和TCGA数据库的差异表达分析，我们预测并随后鉴定了miR-222-3p的关键靶基因。然后，我们使用生物信息学分析探索了关键基因的表达、富集、成对相关性、蛋白质表达、生存分析、主成分分析和免疫意义。本研究表明， 、 、 、 、 、 和 是关键基因。miR-222-3p在THCA组织中高表达，可能导致 和 表达降低和升高。 低表达表明THCA患者预后良好， 高表达表明预后不良。七个关键基因与性别、年龄、种族肿瘤分期和淋巴结转移显著相关。此外，七基因生物标志物具有一定的诊断价值。最后， 表达与免疫细胞密切相关，尤其是B细胞和CD4 T细胞。miR-222-3p及其关键靶基因可能是THCA预后的有前途的生物标志物，并可能成为THCA免疫浸润的关键调节因子。
