Gong Lu, Zhang Xin, Qiu Kai, He Linjuan, Wang Yubo, Yin Jingdong
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Anim Nutr. 2021 Dec;7(4):1115-1123. doi: 10.1016/j.aninu.2021.05.010. Epub 2021 Sep 28.
This study aimed to explore the mechanism underlying arginine-promoted myogenesis of myoblasts. C2C12 cells were cultured with a medium containing 0.1, 0.4, 0.8, or 1.2 mmol/L arginine, respectively. Cell proliferation, viability, differentiation indexes, cytoplasmic Ca concentration, and relative mRNA expression levels of myogenic regulatory factors (MRF) and key Ca channels were measured in the absence or presence of 2 chemical inhibitors, dantrolene (DAN, 10 μmol/L) and nisoldipine (NIS, 10 μmol/L), respectively. Results demonstrated that arginine promoted myogenic differentiation and myotube formation. Compared with the control (0.4 mmol/L arginine), 1.2 mmol/L arginine upregulated the relative mRNA expression levels of myogenin ( and Myomaker at d 2 during myogenic induction ( < 0.05). Cytoplasmic Ca concentrations were significantly elevated by arginine supplementation at d 2 and 4 ( < 0.05). Relative mRNA expression levels of Ca channels including the type 1 ryanodine receptor and voltage-gated Ca channel ( were upregulated by 1.2 mmol/L arginine during 2-d myogenic induction ( < 0.01). However, arginine-promoted myogenic potential of myoblasts was remarkably compromised by DAN and NIS, respectively ( < 0.05). These findings evidenced that the supplementation of arginine promoted myogenic differentiation and myotube formation through increasing cytoplasmic Ca concentration from both extracellular and sarcoplasmic reticulum Ca.
本研究旨在探讨精氨酸促进成肌细胞肌生成的潜在机制。将C2C12细胞分别培养于含有0.1、0.4、0.8或1.2 mmol/L精氨酸的培养基中。在分别存在或不存在两种化学抑制剂丹曲林（DAN，10 μmol/L）和尼索地平（NIS，10 μmol/L）的情况下，测量细胞增殖、活力、分化指标、细胞质Ca浓度以及肌生成调节因子（MRF）和关键Ca通道的相对mRNA表达水平。结果表明，精氨酸促进了肌生成分化和肌管形成。与对照组（0.4 mmol/L精氨酸）相比，1.2 mmol/L精氨酸在成肌诱导第2天时上调了肌细胞生成素（以及成肌生成素）的相对mRNA表达水平（P<0.05）。在第2天和第4天补充精氨酸后，细胞质Ca浓度显著升高（P<0.05）。在2天的成肌诱导过程中，1.2 mmol/L精氨酸上调了包括1型兰尼碱受体和电压门控Ca通道在内的Ca通道的相对mRNA表达水平（P<0.01）。然而，DAN和NIS分别显著削弱了精氨酸促进成肌细胞肌生成的潜力（P<0.05）。这些发现证明，补充精氨酸通过增加细胞外和肌浆网Ca的细胞质Ca浓度来促进肌生成分化和肌管形成。
