Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland.
Department of Laboratory Diagnostics, Medical University of Warsaw, Warsaw, Poland.
PLoS One. 2021 Nov 5;16(11):e0259501. doi: 10.1371/journal.pone.0259501. eCollection 2021.
Serum indoxyl sulfate (IS) levels depend on the production of indole in the gut. The biological effects of IS in the vascular bed could be confirmed by changes in the levels of individual serum proteins during normal pregnancy and in the postpartum period as compared with non-pregnant controls. Albumin (Alb) and α1-acid glycoprotein (AGP, orosomucoid) are the most abundant serum carrier proteins with potential interrelationships with serum levels of IS.
Serum levels of IS, Alb and AGP were measured in 84 pregnant women in the first, second and third trimester of pregnancy and in the postpartum period, as well as in non-pregnant controls (n = 20), using ultra-performance liquid chromatography (UPLC) coupled to mass spectrometry (IS), colorimetric assay (Alb) and immunoturbidimetric assay (AGP).
The postpartum serum levels [mg/L] of IS were lower (p = 0.027) than in the second trimester (mean±SD: 0.85±0.39 vs 0.58±0.32). There were no differences in the IS to ALB ratio calculated in the three trimesters of pregnancy, the postpartum period, and in the non-pregnant controls. The IS/AGP ratio increased from the first to the second trimester (p = 0.039), and decreased in the postpartum period (p<0.05), when it was lower than in the second and third trimester.
The variability of the serum IS/AGP ratio during pregnancy and in the postpartum period may reflect shared involvement in the regulation of their intravascular relationships. The link between serum levels of IS derived from the gut and AGP could serve a potential biomarkers of maternal intestinal metabolism during pregnancy and postpartum.
血清硫酸吲哚酚（IS）水平取决于肠道中吲哚的产生。IS 在血管床中的生物学效应可以通过与非妊娠对照组相比，在正常妊娠和产后期间个体血清蛋白水平的变化来证实。白蛋白（Alb）和α1-酸性糖蛋白（AGP，乳白蛋白）是最丰富的血清载体蛋白，与血清 IS 水平具有潜在的相互关系。
使用超高效液相色谱（UPLC）与质谱联用（IS）、比色法（Alb）和免疫比浊法（AGP）测量 84 名孕妇在妊娠第一、第二和第三孕期以及产后以及 20 名非妊娠对照组（n=20）的血清 IS、Alb 和 AGP 水平。
产后血清 IS [mg/L]水平低于妊娠中期（p=0.027：0.85±0.39 vs 0.58±0.32）。在妊娠的三个孕期、产后和非妊娠对照组中，计算的 IS/Alb 比值没有差异。IS/AGP 比值从第一孕期到第二孕期增加（p=0.039），产后降低（p<0.05），低于第二和第三孕期。
妊娠和产后期间血清 IS/AGP 比值的变化可能反映了它们在血管内关系调节中的共同参与。来自肠道的 IS 与 AGP 之间的血清水平联系可能成为妊娠和产后期间母体肠道代谢的潜在生物标志物。
