Lee Y H, Blakesley R W, Smith L A, Chirikjian J G
Nucleic Acids Res. 1978 Mar;5(3):679-89. doi: 10.1093/nar/5.3.679.
Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzymes. Insolubilization improved thermal stability of Bam HI and Eco RI. The bound enzyme can be recovered from reaction mixtures and reused several times. Upon storage at 4 degrees C, coupled endonucleases remained stable for several months.
II型限制性核酸内切酶Bam HI和Eco RI被共价偶联到琼脂糖上。这些固定化酶产生的几种病毒DNA片段模式与各自游离酶产生的模式相同。酶的结合形式和未结合形式的最佳活性条件相似。固定化提高了Bam HI和Eco RI的热稳定性。结合酶可从反应混合物中回收并重复使用几次。在4℃储存时，偶联的核酸内切酶可保持稳定数月。
