National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
Fish Shellfish Immunol. 2021 Dec;119:635-644. doi: 10.1016/j.fsi.2021.10.005. Epub 2021 Nov 2.
The study investigated the dietary effects of Clostridium autoethanogenum protein (CAP) substituting fish meal on the growth, intestinal histology, serum immune indexes and transcriptome of Pacific white shrimp, Litopenaeus vannamei. Four isonitrogenous and isolipidic diets were designed as the control diet (CON) containing 560 g/kg fish meal, and three fish meal-substituted diets in which 30% (CAP-30), 45% (CAP-45) and 70% (CAP-70) fish meal were replaced with CAP, respectively. The four diets were fed to shrimp with initial body weight of 2.78 ± 0.13 g for 8 weeks. The results showed that the weight gain, feed intake, survival and intestinal villus height in CAP-45 and CAP-70 groups were lower than those of the control and CAP-30 groups (P < 0.05). In addition, the serum aspartate aminotransferase and phenol oxidase activities in all fish meal-substituted groups, and the lysozyme activity in CAP-45 and CAP-70 groups were increased, while the total protein content in CAP-45 and CAP-70 groups was decreased when compared with the control (P < 0.05). Transcriptome profiling of hepatopancreas indicated that high inclusion of CAP negatively affected the protein synthesis and the utilization of nutrients by regulating pancreas secretion, protein digestion and absorption, ribosome pathways, and disturbed the immune system and metabolic processes by phagosomes and lysosomes pathways, thereby affecting the growth performance and immune function of shrimp. In conclusion, CAP could substitute 30% fish meal in a diet containing 560 g/kg fish meal without adverse effects on the growth, intestinal histology and immunity of Pacific white shrimp.
该研究调查了 Clostridium autoethanogenum 蛋白（CAP）替代鱼粉对生长、肠道组织学、血清免疫指标和太平洋白对虾（Litopenaeus vannamei）转录组的影响。设计了四种等氮等脂的饲料作为对照组（CON），含有 560g/kg 鱼粉，以及三种用 CAP 替代 30%（CAP-30）、45%（CAP-45）和 70%（CAP-70）鱼粉的饲料。用初始体重为 2.78±0.13g 的虾投喂这四种饲料 8 周。结果表明，CAP-45 和 CAP-70 组的增重、饲料摄入量、存活率和肠道绒毛高度均低于对照组和 CAP-30 组（P<0.05）。此外，所有鱼粉替代组的血清天冬氨酸转氨酶和酚氧化酶活性，以及 CAP-45 和 CAP-70 组的溶菌酶活性均升高，而 CAP-45 和 CAP-70 组的总蛋白含量则降低与对照组相比（P<0.05）。肝胰腺转录组分析表明，高 CAP 含量通过调节胰腺分泌、蛋白质消化和吸收、核糖体途径，以及通过吞噬体和溶酶体途径扰乱免疫系统和代谢过程，对虾的生长性能和免疫功能产生负面影响，从而负地影响蛋白质合成和营养素的利用。综上所述，CAP 可替代含有 560g/kg 鱼粉的饲料中 30%的鱼粉，对生长、肠道组织学和太平洋白对虾的免疫力无不良影响。
