Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan 430023, China; College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan 430023, China; College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Carbohydr Polym. 2022 Jan 1;275:118673. doi: 10.1016/j.carbpol.2021.118673. Epub 2021 Sep 20.
Chitosan oligosaccharides (COS) are a derivative of low molecular weight chitosan and are potent natural antimicrobial agents. The antimicrobial activity of COS against Aspergillus flavus and Aspergillus fumigatus was evaluated by minimum inhibitory concentration (MIC) and inhibition of mycelial growth. The MICs of COS against these two fungi were 31.2 and 15.6 mg/mL, respectively. COS treatment rendered fungal mycelia wrinkled and deformed with a fractured appearance. COS also increased cellular permeability leading to a significant leakage of cellular components indicating membrane damage. This compound also dose-dependently reduced chitin production and enhanced chitinase activity while enhancing the accumulation of reactive oxygen species (ROS). These characteristics suggested that COS has inhibitory effects against food spoilage fungi and acts on the cell wall and membrane and alters cellular metabolism. COS shows promise for food industry applications since it is non-toxic to higher organisms.
壳寡糖（COS）是低分子量壳聚糖的衍生物，是一种有效的天然抗菌剂。通过最低抑菌浓度（MIC）和抑制菌丝生长来评估 COS 对黄曲霉和烟曲霉的抗菌活性。COS 对这两种真菌的 MIC 分别为 31.2 和 15.6mg/mL。COS 处理使真菌菌丝皱缩变形，呈断裂状。COS 还增加了细胞通透性，导致细胞成分的显著泄漏，表明细胞膜受损。该化合物还剂量依赖性地降低几丁质的产生，增强几丁质酶活性，同时促进活性氧（ROS）的积累。这些特性表明，COS 对食品腐败真菌具有抑制作用，作用于细胞壁和细胞膜并改变细胞代谢。COS 对高等生物无毒，有望在食品工业中得到应用。
