College of Life Science, Institute of Life and Green Development, Hebei University, Baoding 071002, China.
Xiaoshan Donghai Aquaculture Co., Ltd, Xiaoshan 310012, China.
Gene. 2021 Jan 5;764:145098. doi: 10.1016/j.gene.2020.145098. Epub 2020 Aug 27.
Neocaridina denticulate sinensis is a small freshwater economic shrimp, as well as excellent laboratory model for their short life cycle and easy availability. However, the response of N. denticulate sinensis to pervasive copper pollution in aquatic environments has not been deeply investigated yet. Herein, we preformed Illumina sequencing technology to mine the alterations of cephalothorax transcriptome under 2.5 μmol/L of Cu after 48 h. 122,512 unigenes were assembled and 219 unigenes were identified as significantly differentially expressed genes (DEGs) between control and Cu treatment groups. Functional enrichment analysis revealed that DEGs were mostly associated with immune responses and molting, such as endocytosis, Fc gamma R-mediated phagocytosis and chitin metabolic process. Seven genes were chosen for qPCR verification, and the results showed that the transcriptome sequencing data were consistent with the qPCR results. This is the first report of transcriptome information about N. denticulate sinensis. These results provided a direction for the future research of resistance to Cu in this shrimp, and simultaneously enriched gene information of N. denticulate sinensis.
中华锯齿米虾是一种小型淡水经济虾，因其生命周期短、易于获取，而成为优秀的实验室模型。然而，中华锯齿米虾对水生环境中普遍存在的铜污染的反应尚未得到深入研究。在此，我们使用 Illumina 测序技术挖掘了 48 小时后 2.5 μmol/L Cu 处理组中华锯齿米虾头胸甲转录组的变化。共组装了 122512 条 unigenes，其中 219 条 unigenes被鉴定为对照组和 Cu 处理组之间的显著差异表达基因 (DEGs)。功能富集分析表明，DEGs 主要与免疫反应和蜕皮有关，如内吞作用、FcγR 介导的吞噬作用和几丁质代谢过程。选择了 7 个基因进行 qPCR 验证，结果表明转录组测序数据与 qPCR 结果一致。这是关于中华锯齿米虾转录组信息的首次报道。这些结果为该虾未来对 Cu 的抗性研究提供了方向，同时丰富了中华锯齿米虾的基因信息。
