Kangiella shandongensis sp. nov., a novel species isolated from saltern in Yantai, China.

A Gram-stain-negative, wheat, rod-shaped, non-motile, non-spore forming, and facultatively anaerobic bacterium strain, designated as PI, was isolated from saline silt samples collected in saltern in Yantai, Shandong, China. Growth was observed within the ranges 4-45 °C (optimally at 33 °C), pH 6.0-9.0 (optimally at pH 7.0) and 1.0-11.0% NaCl (optimally at 3.0%, w/v). Strain PI showed highest 16S rRNA gene sequence similarity to Kangiella sediminilitoris BB-Mw22 (98.3%) and Kangiella taiwanensis KT1 (98.3%). The major cellular fatty acids (> 10% of the total fatty acids) were iso-C (52.7%) and summed featured 9 (iso-Cω9c/C 10-methyl, 11.8%). The major polar lipids identified were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol. The major respiratory isoprenoid quinone was Q-8. The G + C content of the genomic DNA was 45.8%. Average Nucleotide Identity values between whole genome sequences of strain PI and next related type strains supported the novel species status. Based on physiological, biochemical, chemotaxonomic characteristics and genomic analysis, strain PI is considered to represent a novel species within the genus Kangiella, for which the name Kangiella shandongensis sp. nov. is proposed. The type strain is PI (= KCTC 82509 = MCCC 1K04352).