Marine College, Shandong University, Weihai, 264209, China.
Antonie Van Leeuwenhoek. 2022 Jan;115(1):33-40. doi: 10.1007/s10482-021-01682-9. Epub 2021 Nov 7.
A Gram-stain-negative, wheat, rod-shaped, non-motile, non-spore forming, and facultatively anaerobic bacterium strain, designated as PI, was isolated from saline silt samples collected in saltern in Yantai, Shandong, China. Growth was observed within the ranges 4-45 °C (optimally at 33 °C), pH 6.0-9.0 (optimally at pH 7.0) and 1.0-11.0% NaCl (optimally at 3.0%, w/v). Strain PI showed highest 16S rRNA gene sequence similarity to Kangiella sediminilitoris BB-Mw22 (98.3%) and Kangiella taiwanensis KT1 (98.3%). The major cellular fatty acids (> 10% of the total fatty acids) were iso-C (52.7%) and summed featured 9 (iso-Cω9c/C 10-methyl, 11.8%). The major polar lipids identified were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol. The major respiratory isoprenoid quinone was Q-8. The G + C content of the genomic DNA was 45.8%. Average Nucleotide Identity values between whole genome sequences of strain PI and next related type strains supported the novel species status. Based on physiological, biochemical, chemotaxonomic characteristics and genomic analysis, strain PI is considered to represent a novel species within the genus Kangiella, for which the name Kangiella shandongensis sp. nov. is proposed. The type strain is PI (= KCTC 82509 = MCCC 1K04352).
一株革兰氏阴性、小麦形、杆状、非运动、非孢子形成、兼性厌氧细菌菌株，被命名为 PI，从中国山东烟台盐田采集的盐泥样本中分离得到。在 4-45°C（最佳温度为 33°C）、pH 值 6.0-9.0（最佳 pH 值为 7.0）和 1.0-11.0%NaCl（最佳 3.0%，w/v）的范围内观察到生长。菌株 PI 与 Kangiella sediminilitoris BB-Mw22（98.3%）和 Kangiella taiwanensis KT1（98.3%）的 16S rRNA 基因序列相似度最高。主要细胞脂肪酸（占总脂肪酸的>10%）为 iso-C（52.7%）和特征脂肪酸 9（iso-Cω9c/C 10-甲基，11.8%）。鉴定出的主要极性脂类为双磷脂酰甘油、磷脂酰乙醇胺、磷脂单甲基乙醇胺和磷脂酰甘油。主要呼吸异戊二烯醌为 Q-8。基因组 DNA 的 G+C 含量为 45.8%。菌株 PI 的全基因组序列与下一相关模式菌株之间的平均核苷酸同一性值支持了新物种的地位。基于生理、生化、化学生态特征和基因组分析，菌株 PI 被认为代表了 Kangiella 属中的一个新种，建议将其命名为 Kangiella shandongensis sp. nov.。模式菌株为 PI（=KCTC 82509=MCCC 1K04352）。
