Xu Fang-di, Li Xue-Gong, Xiao Xiang, Xu Jun
State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Institute of Oceanology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2315-2319. doi: 10.1099/ijs.0.000257. Epub 2015 Apr 13.
A taxonomic study employing a polyphasic approach was carried out on strain FT102(T), which was isolated from a deep-sea sediment sample collected in the south-west Indian Ocean at a depth of 2784 m. The strain was Gram-stain-negative, non-motile, rod-shaped and non-spore-forming. It grew optimally at 37-42 °C, pH 6.5-8.5 and in the presence of 1-4% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences confirmed the separation of the novel strain from recognized members of the genus Kangiella that are available in public databases. Strain FT102(T) exhibited 95.5-98.6% 16S rRNA gene sequence similarity to the type strains of the eight recognized species of the genus Kangiella. The chemotaxonomically characteristic fatty acid iso-C15:0 and ubiquinone Q-8 were also detected. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G + C content of strain FT102(T) was 45.0 mol%. The mean DNA-DNA relatedness values between strain FT102(T) and the type strains of Kangiella aquimarina and Kangiella koreensis were 47.3% and 13.7%, respectively. The combined results of phylogenetic, physiological and chemotaxonomic studies indicated that strain FT102(T) was affiliated with the genus Kangiella but differed from the recognized species of the genus Kangiella. Therefore, strain FT102T represents a novel species of the genus Kangiella, for which the name Kangiella profundi sp. nov. is proposed. The type strain is FT102(T) ( = CGMCC 1.12959(T) = KCTC 42297(T) = JCM 30232(T)).
采用多相分类方法对菌株FT102(T)进行了分类学研究，该菌株是从西南印度洋2784米深处采集的深海沉积物样本中分离得到的。该菌株革兰氏染色阴性，无运动性，杆状，不产芽孢。在37-42℃、pH 6.5-8.5以及1-4%（w/v）NaCl存在的条件下生长最佳。对16S rRNA基因序列的系统发育分析证实，该新菌株与公共数据库中已确认的康氏菌属成员分离开来。菌株FT102(T)与康氏菌属八个已确认物种的模式菌株的16S rRNA基因序列相似性为95.5-98.6%。还检测到化学分类学特征性脂肪酸异-C15:0和泛醌Q-8。主要极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰单甲基乙醇胺。菌株FT102(T)的DNA G + C含量为45.0 mol%。菌株FT102(T)与海洋康氏菌和韩国康氏菌模式菌株之间的平均DNA-DNA相关性值分别为47.3%和13.7%。系统发育、生理和化学分类学研究的综合结果表明，菌株FT102(T)属于康氏菌属，但与康氏菌属已确认的物种不同。因此，菌株FT102T代表康氏菌属的一个新物种，为此提出新名称深海康氏菌（Kangiella profundi sp. nov.）。模式菌株为FT102(T)（= CGMCC 1.12959(T) = KCTC 42297(T) = JCM 30232(T)）。
