Kim Jong-Hwa, Ward Alan C, Kim Wonyong
Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea.
Antonie Van Leeuwenhoek. 2015 May;107(5):1291-8. doi: 10.1007/s10482-015-0423-5. Epub 2015 Mar 8.
A Gram-negative bacterium, designated CAU 1040(T), which was isolated from marine sand obtained from Jeju Island in South Korea, was characterized as an aerobic rod-shaped organism that that was non-motile, non-spore-forming and halophilic. The bacterium grew optimally at 37 °C, at pH 8, and in the presence of 2% (w/v) NaCl. The taxonomic classification of CAU 1040(T) was investigated using a polyphasic characterization approach. While phylogenetic analysis of the 16S rRNA gene sequence revealed that CAU 1040(T) belongs to the genus Kangiella, the strain exhibited only 94.4-95.4% sequence similarity to the previously described Kangiella species. Similar to other Kangiella species, Q-8 was the predominant ubiquionone and iso-C(15:0) was the major cellular fatty acid detected in strain CAU 1040(T). The predominant polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The G+C content of the CAU 1040(T) genome was 45.3 mol%. The phylogenetic, physiological, biochemical and chemotaxonomic data obtained in this study indicate that strain CAU 1040(T) represents a novel species of the genus Kangiella, for which the name Kangiella chungangensis sp. nov. is hereby proposed. The type strain is CAU 1040(T) (KCTC 42299(T), NBRC 110728(T)).
从韩国济州岛的海砂中分离出的一株革兰氏阴性菌，命名为CAU 1040(T)，其特征为需氧的杆状菌，不运动，不产芽孢且嗜盐。该细菌在37℃、pH 8以及2%（w/v）NaCl存在的条件下生长最佳。采用多相分类法对CAU 1040(T)进行分类学研究。16S rRNA基因序列的系统发育分析表明，CAU 1040(T)属于康氏菌属，但该菌株与先前描述的康氏菌属物种的序列相似性仅为94.4 - 95.4%。与其他康氏菌属物种相似，Q - 8是主要的泛醌，异C(15:0)是在CAU 1040(T)菌株中检测到的主要细胞脂肪酸。鉴定出的主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。CAU 1040(T)基因组的G + C含量为45.3 mol%。本研究获得的系统发育、生理、生化和化学分类学数据表明，CAU 1040(T)菌株代表康氏菌属的一个新物种，为此提出新名称康氏中江菌（Kangiella chungangensis sp. nov.）。模式菌株为CAU 1040(T)（KCTC 42299(T)，NBRC 110728(T)）。
