Laboratory of Chemical Biology, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Laboratory of Chemical Biology, Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Brain Res. 2021 Dec 15;1773:147704. doi: 10.1016/j.brainres.2021.147704. Epub 2021 Oct 29.
Among the most studied diseases that affect the central nervous system are Parkinson's, Alzheimer's, and Huntington's diseases, but the lack of effective biomarkers, accurate diagnosis, and precise treatment for each of them is currently an issue. Due to the contribution of biomarkers in supporting diagnosis, many recent efforts have focused on their identification and validation at the beginning or during the progression of the mental illness. Metabolome reveals the metabolic processes that result from protein activities under the guided gene expression and environmental factors, either in healthy or pathological conditions. In this context, metabolomics has proven to be a valuable approach. Currently, magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) are the most commonly used bioanalytical techniques for metabolomics. MS-assisted profiling is considered the most versatile technique, and the NMR is the most reproductive. However, each one of them has its drawbacks. In this review, we summarized several alterations in metabolites that have been reported for these three classic brain diseases using MS and NMR-based research, which might suggest some possible biomarkers to support the diagnosis and/or new targets for their treatment.
在影响中枢神经系统的最常见疾病中，有帕金森病、阿尔茨海默病和亨廷顿病，但目前缺乏针对每种疾病的有效生物标志物、准确诊断和精确治疗方法。由于生物标志物在支持诊断方面的作用，最近许多研究都集中在精神疾病的早期或进展过程中对其进行鉴定和验证。代谢组学揭示了在指导基因表达和环境因素的作用下，蛋白质活动导致的代谢过程，无论是在健康还是病理条件下。在这种情况下，代谢组学已被证明是一种很有价值的方法。目前，磁共振波谱（NMR）和质谱（MS）是代谢组学中最常用的生物分析技术。MS 辅助分析被认为是最通用的技术，而 NMR 则是最具重复性的技术。但是，它们各自都有缺点。在这篇综述中，我们总结了使用 MS 和基于 NMR 的研究报道的这三种经典脑部疾病的几种代谢物的变化，这可能提示了一些可能的生物标志物，以支持诊断和/或为它们的治疗提供新的靶点。
