Wang Hailin, Lu Wei, Ke Lijing, Wang Yi, Zhou Jianwu, Rao Pingfan
Food Nutrition Science Centre, School of Food Science and Biotechnology, Zhejiang Gongshang University, 1st Lab Building, #149 Jiaogong Road, Xihu District, Hangzhou 310012, Zhejiang, China.
Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Colloids Surf A Physicochem Eng Asp. 2022 Jan 20;633:127849. doi: 10.1016/j.colsurfa.2021.127849. Epub 2021 Nov 3.
Hydroxychloroquine sulfate (HCQ) is a well-established antimalarial drug that has received considerable attention during the COVID-19 associated pneumonia epidemic. Gelatin is a multifunctional biomacromolecule with pharmaceutical applications and can be used to deliver HCQ. The effect of HCQ on the gelation behaviors, water mobility, and structure of gelatin was investigated to understand the interaction between the drug and its delivery carrier. The gel strength, hardness, gelling (T) and melting (T) temperatures, gelation rate (k), and water mobility of gelatin decreased with increasing amounts of HCQ. The addition of HCQ led to hydrogen bonding that interfered with triple helix formation in gelatin. Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD) analysis further confirmed that the interaction between HCQ and gelatin is primarily through hydrogen bonding. Atomic force microscopy (AFM) revealed that higher content of HCQ resulted in more and larger aggregates in gelatin. These results provide not only an important understanding of gelatin for drug delivery design but also a basis for the studying interactions between a drug and its delivery carrier.
硫酸羟氯喹（HCQ）是一种成熟的抗疟药物，在新型冠状病毒肺炎疫情期间受到了广泛关注。明胶是一种具有药物应用的多功能生物大分子，可用于递送HCQ。研究了HCQ对明胶的凝胶化行为、水流动性和结构的影响，以了解药物与其递送载体之间的相互作用。随着HCQ含量的增加，明胶的凝胶强度、硬度、胶凝温度（T）和熔化温度（T）、凝胶化速率（k）以及水流动性均降低。HCQ的加入导致氢键形成，干扰了明胶中三螺旋的形成。傅里叶变换红外光谱（FTIR）和X射线衍射仪（XRD）分析进一步证实，HCQ与明胶之间的相互作用主要是通过氢键。原子力显微镜（AFM）显示，较高含量的HCQ导致明胶中形成更多更大的聚集体。这些结果不仅为药物递送设计中对明胶的重要理解提供了依据，也为研究药物与其递送载体之间的相互作用奠定了基础。
