将植物乳杆菌和 DHA 脂肪酸共微胶囊包埋于藻酸盐-果胶-明胶生物复合材料中。
Co-microencapsulation of Lactobacillus plantarum and DHA fatty acid in alginate-pectin-gelatin biocomposites.
机构信息
Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box: 11365-11155, Tehran, Iran.
Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14155-4838, Tehran, Iran.
出版信息
Carbohydr Polym. 2018 Nov 1;199:266-275. doi: 10.1016/j.carbpol.2018.07.002. Epub 2018 Jul 10.
The aim of this study was to investigate the co-microencapsulation of Lactobacillus plantarum and DHA-rich oil in a novel gastrointestinal-resistant biocomposite composed of alginate, pectin and gelatin. The optimal biocomposite consisted of 1.06% alginate, 0.55% pectin and 0.39% gelatin showed 88.66% survivability of the microencapsulated cells compared to the free cells (50.36%). In addition, co-microencapsule containing probiotic and DHA fatty acid was synthesized and physicochemically analyzed using SEM, FTIR, TGA, XRD. The results from SEM clearly confirmed that cells were completely entrapped in the matrix and DHA increased smoothness and compactness of the surface of the particles. FTIR spectra revealed the formation of hydrogen and Van der Waals bonds between macromolecules and the core materials. X-ray pattern of co-microencapsules identified amorphous structure compared to capsules containing only DHA or probiotic. TGA analysis revealed the thermal stability of DHA-loaded capsules compared to un-loaded ones.
本研究旨在探索将植物乳杆菌和富含 DHA 的油共微囊化于由海藻酸钠、果胶和明胶组成的新型胃肠道抵抗性生物复合材料中。最优的生物复合材料由 1.06%的海藻酸钠、0.55%的果胶和 0.39%的明胶组成，与游离细胞（50.36%）相比，微囊化细胞的存活率达到 88.66%。此外，还通过 SEM、FTIR、TGA、XRD 对含有益生菌和 DHA 脂肪酸的共微囊进行了物理化学分析。SEM 的结果清楚地证实，细胞完全被包埋在基质中，而 DHA 增加了颗粒表面的光滑度和致密性。FTIR 光谱显示大分子与芯材之间形成了氢键和范德华键。共微囊的 X 射线图谱与仅含有 DHA 或益生菌的胶囊相比，确定为无定形结构。TGA 分析表明，与未加载的胶囊相比，DHA 负载的胶囊具有更好的热稳定性。
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