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使用前端微流变学对β地中海贫血特征和缺铁性贫血进行血液流变学表征

Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry.

作者信息

Méndez-Mora Lourdes, Cabello-Fusarés Maria, Ferré-Torres Josep, Riera-Llobet Carla, Krishnevskaya Elena, Trejo-Soto Claudia, Payán-Pernía Salvador, Hernández-Rodríguez Inés, Morales-Indiano Cristian, Alarcón Tomas, Vives-Corrons Joan-Lluis, Hernandez-Machado Aurora

机构信息

Department of Condensed Matter Physics, University of Barcelona, Barcelona, Spain.

Centre de Recerca Matemàtica, Barcelona, Spain.

出版信息

Front Physiol. 2021 Oct 21;12:761411. doi: 10.3389/fphys.2021.761411. eCollection 2021.

DOI:10.3389/fphys.2021.761411
PMID:34744796
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8566979/
Abstract

The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of β-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, β-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA).

摘要

这项工作的目的是通过对不同献血者全血样本的流变学特征分析，开发一种与血细胞比容无关的方法来检测β地中海贫血特征（β-TT）和缺铁性贫血（IDA）。本文所获得的结果是开发一种用于诊断和临床随访患有血液系统疾病和红细胞（RBC）形态改变的β-TT患者的前微流变床旁检测设备的基础。通过检测样本流体-空气界面在微流控通道中的推进速度，将粘度计算为平均前沿速度的函数。健康献血者、β-TT和IDA样本获得了不同的粘度曲线。引入了一个数学模型来比较不同血细胞比容的样本，根据血液的健康状况对粘度曲线模式进行分类。在特定剪切速率值下，流体的粘度会因多种红细胞因素而变化，如形状和大小、血红蛋白（Hb）含量、膜刚性和血细胞比容浓度。使用健康献血者的血液和血浆作为参考。为了验证其作为诊断工具的潜在临床价值，将粘度结果与通过红细胞变形性评估的金标准方法——激光光学旋转红细胞分析仪（LoRRCA）获得的结果进行比较。

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An Integrated Detection Method for Flow Viscosity Measurements in Microdevices.微器件中流动粘度测量的集成检测方法。
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