Sriramoju Vidyasagar, Alfano Robert R
J Biomed Opt. 2015 Oct;20(10):108001. doi: 10.1117/1.JBO.20.10.108001.
Femtosecond (fs) pulse lasers in the near-infrared (NIR) range exhibit very distinct properties upon their interaction with biomolecules compared to the corresponding continuous wave (CW) lasers. Ultrafast NIR laser tissue bonding (LTB) was used to fuse edges of two opposing animal tissue segments in vivo using fs laser photoexcitation of the native vibrations of chomophores. The fusion of the incised tissues was achieved in vivo at the molecular level as the result of the energy–matter interactions of NIR laser radiation with water and the structural proteins like collagen in the target tissues. Nonthermal vibrational excitation from the fs laser absorption by water and collagen induced the formation of cross-links between tissue proteins on either sides of the weld line resulting in tissue bonding. No extrinsic agents were used to facilitate tissue bonding in the fs LTB. These studies were pursued for the understanding and evaluation of the role of ultrafast NIR fs laser radiation in the LTB and consequent wound healing. The fs LTB can be used for difficult to suture structures such as blood vessels, nerves, gallbladder, liver, intestines, and other viscera. Ultrafast NIR LTB yields promising outcomes and benefits in terms of wound closure and wound healing under optimal conditions.
与相应的连续波(CW)激光相比,近红外(NIR)范围内的飞秒(fs)脉冲激光在与生物分子相互作用时表现出非常独特的特性。超快近红外激光组织黏合(LTB)通过对发色团的固有振动进行飞秒激光光激发,在体内融合两个相对的动物组织片段的边缘。由于近红外激光辐射与靶组织中的水和胶原蛋白等结构蛋白之间的能量 - 物质相互作用,在体内分子水平上实现了切割组织的融合。水和胶原蛋白对飞秒激光的吸收产生的非热振动激发导致熔合线两侧的组织蛋白之间形成交联,从而实现组织黏合。在飞秒激光组织黏合过程中未使用外部试剂来促进组织黏合。进行这些研究是为了理解和评估超快近红外飞秒激光辐射在激光组织黏合及随后伤口愈合中的作用。飞秒激光组织黏合可用于血管、神经、胆囊、肝脏、肠道和其他内脏等难以缝合的结构。在最佳条件下,超快近红外激光组织黏合在伤口闭合和伤口愈合方面产生了有前景的结果和益处。