NUS Environmental Research Institute, National University of Singapore, 1 Create Way, Create Tower #15-02, Singapore 138602, Singapore; Energy and Environmental Sustainability for Megacities (E2S2) Phase II, Campus for Research Excellence and Technological Enterprise (CREATE), 1 CREATE Way, Singapore 138602, Singapore.
Korea Biochar Research Center & Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
Bioresour Technol. 2022 Jan;344(Pt B):126294. doi: 10.1016/j.biortech.2021.126294. Epub 2021 Nov 5.
The enhanced production of microbial lipids suitable for manufacturing biodiesel from oleaginous yeast Lipomyces starkeyi is critically reviewed. Recent advances in several aspects involving the biosynthetic pathways of lipids, current conversion efficiencies using various carbon sources, intensification strategies for improving lipid yield and productivity in L. starkeyi fermentation, and lipid extraction approaches are analyzed from about 100 papers for the past decade. Key findings on strategies are summarized, including (1) optimization of parameters, (2) cascading two-stage systems, (3) metabolic engineering strategies, (4) mutagenesis followed by selection, and (5) co-cultivation of yeast and algae. The current technical limitations are analyzed. Research suggestions like examination of more gene targets via metabolic engineering are proposed. This is the first comprehensive review on the latest technical advances in strategies from the perspective of process and metabolic engineering to further increase the lipid yield and productivity from L. starkeyi fermentation.
从产油酵母拉斯克氏油脂酵母（Lipomyces starkeyi）中提取适合生产生物柴油的微生物油脂的强化生产受到了严格的审查。本文对过去十年中约 100 篇论文中涉及油脂生物合成途径、利用各种碳源的当前转化效率、提高拉斯克氏油脂酵母发酵中油脂产量和生产力的强化策略以及油脂提取方法的几个方面的最新进展进行了分析。总结了关键发现的策略，包括（1）优化参数，（2）两阶段级联系统，（3）代谢工程策略，（4）诱变后选择，（5）酵母和藻类的共培养。分析了当前的技术限制，并提出了研究建议，例如通过代谢工程检查更多的基因靶标。这是第一篇从过程和代谢工程的角度对提高拉斯克氏油脂酵母发酵中油脂产量和生产力的策略的最新技术进展进行综合审查的文章。
