DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA.
Appl Microbiol Biotechnol. 2022 Sep;106(17):5629-5642. doi: 10.1007/s00253-022-12084-w. Epub 2022 Jul 30.
Oleaginous yeasts have received significant attention due to their substantial lipid storage capability. The accumulated lipids can be utilized directly or processed into various bioproducts and biofuels. Lipomyces starkeyi is an oleaginous yeast capable of using multiple plant-based sugars, such as glucose, xylose, and cellobiose. It is, however, a relatively unexplored yeast due to limited knowledge about its physiology. In this study, we have evaluated the growth of L. starkeyi on different sugars and performed transcriptomic and metabolomic analyses to understand the underlying mechanisms of sugar metabolism. Principal component analysis showed clear differences resulting from growth on different sugars. We have further reported various metabolic pathways activated during growth on these sugars. We also observed non-specific regulation in L. starkeyi and have updated the gene annotations for the NRRL Y-11557 strain. This analysis provides a foundation for understanding the metabolism of these plant-based sugars and potentially valuable information to guide the metabolic engineering of L. starkeyi to produce bioproducts and biofuels. KEY POINTS: • L. starkeyi metabolism reprograms for consumption of different plant-based sugars. • Non-specific regulation was observed during growth on cellobiose. • L. starkeyi secretes β-glucosidases for extracellular hydrolysis of cellobiose.
由于其大量储存脂质的能力，油脂酵母受到了极大的关注。积累的脂质可以直接利用或加工成各种生物制品和生物燃料。粘红酵母是一种能够利用多种植物源糖（如葡萄糖、木糖和纤维二糖）的油脂酵母。然而，由于对其生理学的了解有限，它仍然是一种相对未被探索的酵母。在这项研究中，我们评估了粘红酵母在不同糖上的生长情况，并进行了转录组和代谢组分析，以了解糖代谢的潜在机制。主成分分析显示，不同糖生长导致的差异明显。我们进一步报告了在这些糖上生长时激活的各种代谢途径。我们还观察到粘红酵母中的非特异性调节，并更新了 NRRL Y-11557 菌株的基因注释。这项分析为理解这些植物源糖的代谢提供了基础，并为指导粘红酵母生产生物制品和生物燃料的代谢工程提供了有价值的信息。关键点：
粘红酵母的代谢为消耗不同的植物源糖而重新编程。
在纤维二糖上生长时观察到非特异性调节。
粘红酵母分泌β-葡萄糖苷酶用于纤维二糖的细胞外水解。
