Arends J W, Schutte B, Wiggers T, Verstijnen C P, Blijham G H, Bosman F T
Cancer Res. 1987 Aug 15;47(16):4342-4.
In order to test the contention that metastasis is a selective process and that therefore metastases might show a more restricted pattern of phenotypic and genotypic characteristics than primary tumors, we compared the expression of carcinoembryonic antigen, Ca 19-9, secretory component, serotonin, and mucin production as well as flow cytometric data on DNA content and percentage of S-phase cells in 87 primary large bowel carcinomas and their lymph node metastases. In a majority of the cases primary tumors and their metastases were largely identical with regard to the examined phenotypic features. In discrepant cases, however, metastases did not invariably show a more restricted pattern than primary tumors, indicating high differentiational plasticity of primary and metastatic colorectal cancer cells. In contrast, in a number of cases genotypic discrepancies were observed. We conclude that phenotypic characteristics of colorectal cancer cells cannot be used to study the pathogenesis of lymph node metastasis. Genotypic studies, however, suggest that lymphogenic metastasis may be a selective event.
为了验证转移是一个选择性过程这一论点，即转移灶可能比原发性肿瘤表现出更有限的表型和基因型特征模式，我们比较了87例原发性大肠癌及其淋巴结转移灶中癌胚抗原、Ca 19 - 9、分泌成分、血清素和粘蛋白的产生情况，以及DNA含量和S期细胞百分比的流式细胞术数据。在大多数病例中，原发性肿瘤及其转移灶在所检测的表型特征方面基本相同。然而，在存在差异的病例中，转移灶并不总是比原发性肿瘤表现出更有限的模式，这表明原发性和转移性结肠直肠癌细胞具有高度的分化可塑性。相比之下，在一些病例中观察到了基因型差异。我们得出结论，结肠直肠癌细胞的表型特征不能用于研究淋巴结转移的发病机制。然而，基因型研究表明，淋巴转移可能是一个选择性事件。
