Takeuchi K, Ohno T, Okabe S
Dig Dis Sci. 1987 Aug;32(8):889-96. doi: 10.1007/BF01296714.
Exposure of the stomach for 30 min to acidified sodium taurocholate (TC) (1-20 mM) or sodium salicylate (SA) (10-80 mM) caused a reduction of transmucosal PD and an increase of luminal pH in anesthetized rats, in a concentration-related manner. Acidified aspirin (ASA) (10-80 mM) reduced PD in the same manner, without significant effect on pH. Histologically, these agents similarly produced damage to the surface cells. After a 30-min exposure to either 20 mM TC or 40 mM SA, acid secretion ceased and bicarbonate (0.5-1 mumol/10 min) appeared in the lumen, whereas acid secretion persisted in the stomach exposed to 40 mM ASA. However, under cimetidine infusion (8 mg/kg/hr) these agents produced similar degrees of luminal alkalinization (approximately 1 mumol/10 min). Pretreatment with indomethacin (5 mg/kg, subcutaneously) significantly inhibited the increase of pH seen after exposure to 20 mM TC, but had no effect on the increase of pH caused by 40 mM SA. Concurrent administration of 16,16-dmPGE2 (3 micrograms/kg, subcutaneously) significantly antagonized the effect of indomethacin in the stomach exposed to 20 mM TC and even increased the pH in the stomach exposed to 40 mM ASA. After a 3-hr exposure to these agents, there was macroscopically apparent damage only in the stomach exposed to ASA, although the PD was similarly reduced in response to either agent. The levels of PGE2 in the corpus mucosa were significantly increased in stomachs exposed to 20 mM TC and 40 mM SA, but decreased in those exposed to 40 mM ASA.(ABSTRACT TRUNCATED AT 250 WORDS)
在麻醉大鼠中，将胃暴露于酸化牛磺胆酸钠（TC）（1 - 20 mM）或水杨酸钠（SA）（10 - 80 mM）30分钟，会导致跨粘膜电位差（PD）降低以及管腔pH值升高，且呈浓度相关。酸化阿司匹林（ASA）（10 - 80 mM）以相同方式降低PD，但对pH值无显著影响。组织学上，这些药物同样对表面细胞造成损伤。暴露于20 mM TC或40 mM SA 30分钟后，胃酸分泌停止，管腔中出现碳酸氢盐（0.5 - 1 μmol/10分钟），而暴露于40 mM ASA的胃中胃酸分泌持续存在。然而，在西咪替丁输注（8 mg/kg/小时）情况下，这些药物产生相似程度的管腔碱化（约1 μmol/10分钟）。吲哚美辛预处理（5 mg/kg，皮下注射）显著抑制暴露于20 mM TC后观察到的pH值升高，但对40 mM SA引起的pH值升高无影响。同时给予16,16 - 二甲基前列腺素E2（3 μg/kg，皮下注射）显著拮抗吲哚美辛在暴露于20 mM TC的胃中的作用，甚至使暴露于40 mM ASA的胃中的pH值升高。暴露于这些药物3小时后，宏观上仅在暴露于ASA的胃中出现明显损伤，尽管对任何一种药物反应时PD同样降低。暴露于20 mM TC和40 mM SA的胃体粘膜中前列腺素E2水平显著升高，但暴露于40 mM ASA的胃中则降低。（摘要截断于250字）
