Ligumsky M, Grossman M I, Kauffman G L
Am J Physiol. 1982 Apr;242(4):G337-41. doi: 10.1152/ajpgi.1982.242.4.G337.
These studies were designed to determine the role of endogenous gastric mucosal prostaglandins (PG) in maintaining mucosal integrity. Vagally denervated, separated pouches of gastric fundic mucosa in unanesthetized dogs were irrigated with either acetylsalicylic acid (ASA) or salicylic acid (SA) (0, 2.5, 5.0, 10.0, 20.0, and 40.0 mM) in 150 mM HCl. Transmucosal potential difference (PD) and net H+, Na+, and K+ flux were measured. Mucosal ex vivo generation of 6-oxo-PGF1 alpha, PGE2, and PGF2 alpha was measured by radioimmunoassay in mucosal biopsies taken after exposure to each agent. No difference in PD or net H+, Na+, or K+ flux was observed between pouch irrigation with ASA or SA at 2.5-20.0 mM concentrations. Net H+ and Na+ flux was significantly greater (P less than 0.01) after irrigation with 40 mM SA than with 40 mM ASA. No significant reduction in gastric mucosal ex vivo generation of 6-oxo-PGF1 alpha (range, 65-98 ng.g-.min-1), PGE2 (range, 250-326 ng.g-1.min-1), or PGF2 alpha (range, 115-156 ng.g-1.min-1) was observed after pouch irrigation with all concentrations of SA. In comparison, gastric mucosal ex vivo generation of 6-oxo-PGF1 alpha (range, 75-2 ng.g-1.min-1), PGE2 (range, 22-3 ng.g-1.min-1), and PGF2 alpha (range, 40-2 ng.g-1.min-1) was significantly reduced after irrigation with all concentrations of ASA. From these data, we conclude that the activity of endogenous gastric prostacyclin, PGE2 alpha, and PGF2 alpha is not a prerequisite for mucosal integrity as measured by PD and net cationic flux.
这些研究旨在确定内源性胃黏膜前列腺素（PG）在维持黏膜完整性中的作用。在未麻醉的犬身上，将胃底黏膜的迷走神经切断并分离成小袋，用150 mM盐酸中的乙酰水杨酸（ASA）或水杨酸（SA）（0、2.5、5.0、10.0、20.0和40.0 mM）进行灌洗。测量跨黏膜电位差（PD）以及净H⁺、Na⁺和K⁺通量。在暴露于每种药物后，通过放射免疫分析法在黏膜活检组织中测量黏膜离体产生的6 - 氧代 - PGF1α、PGE2和PGF2α。在2.5 - 20.0 mM浓度下，用ASA或SA灌洗小袋之间，未观察到PD或净H⁺、Na⁺或K⁺通量的差异。用40 mM SA灌洗后，净H⁺和Na⁺通量显著大于（P < 0.01）用40 mM ASA灌洗后。在用所有浓度的SA灌洗小袋后，未观察到胃黏膜离体产生的6 - 氧代 - PGF1α（范围为65 - 98 ng·g⁻¹·min⁻¹）、PGE2（范围为250 - 326 ng·g⁻¹·min⁻¹）或PGF2α（范围为115 - 156 ng·g⁻¹·min⁻¹）有显著降低。相比之下，在用所有浓度的ASA灌洗后，胃黏膜离体产生的6 - 氧代 - PGF1α（范围为75 - 2 ng·g⁻¹·min⁻¹）、PGE2（范围为22 - 3 ng·g⁻¹·min⁻¹）和PGF2α（范围为40 - 2 ng·g⁻¹·min⁻¹）显著降低。根据这些数据，我们得出结论，内源性胃前列环素、PGE2α和PGF2α的活性并非通过PD和净阳离子通量所测量的黏膜完整性的先决条件。
