Kos Z, Pavelić L, Pekić B, Pavelić K
Oncology. 1987;44(4):245-7. doi: 10.1159/000226487.
The activity and intracellular distribution of catalase was studied in culture human myeloid leukemia cells before and after induction of differentiation with tunicamycin. Activity of catalase was increased 5-fold in acute myeloid leukemia cells (AML) and 3-fold in chronic myeloid leukemia cells in comparison with normal granulocytes. Tunicamycin induced differentiation of HL-60 line and primary AML line characterized by increase in phagocytic cells and changes to resemble mature myeloid cells. Fc receptors were also induced in cells after tunicamycin treatment. Induction of differentiation with tunicamycin decreased high activity of catalase in cultured leukemic cells. The results of digitonin titration experiments showed that in control granulocytes and differentiated leukemic cells most of the catalase activity is present in subcellular particles distinct from mitochondria or lysosomes. In contrast, the catalase activity in undifferentiated cells is present in the same compartment as the other cytosolic markers.
在用衣霉素诱导分化前后，对培养的人髓系白血病细胞中过氧化氢酶的活性及细胞内分布进行了研究。与正常粒细胞相比，急性髓系白血病细胞（AML）中过氧化氢酶的活性增加了5倍，慢性髓系白血病细胞中增加了3倍。衣霉素诱导HL-60细胞系和原发性AML细胞系分化，其特征为吞噬细胞增加，并转变为类似成熟髓系细胞的形态。衣霉素处理后的细胞中也诱导产生了Fc受体。衣霉素诱导分化降低了培养白血病细胞中过氧化氢酶的高活性。洋地黄皂苷滴定实验结果表明，在对照粒细胞和分化的白血病细胞中，大部分过氧化氢酶活性存在于与线粒体或溶酶体不同的亚细胞颗粒中。相反，未分化细胞中的过氧化氢酶活性与其他胞质标记物存在于同一区室中。
