Bickford-Wimer P, Granholm A C, Bygdeman M, Hoffer B, Olson L, Seiger A, Strömberg I
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5957-61. doi: 10.1073/pnas.84.16.5957.
Human fetal tissue fragments from cortex cerebri and cerebellum were grafted to the anterior chamber of the eye of adult athymic nude rats. The grafts were obtained from tissue fragments recovered after elective routine abortions, performed in weeks 8-11 of gestation. Both cerebellar and cortex cerebri grafts survived and developed in the anterior chamber of the eye for 1-4 months. The transplants slowly became vascularized from the host iris. The grafts developed blood vessels with laminin-immunoreactive walls and contained relatively high amounts of glial fibrillary acidic protein- and neurofilament-immunoreactivity in the neuropil after 4 months in oculo. Recordings of extracellular action potentials from the grafts revealed spontaneously active neurons with action-potential waveforms similar to those observed in immature rodents. Morphologically, the grafts showed no signs of rejection. Clusters and bands of large neurons resembling Purkinje cells and dense aggregates of smaller granule-like cells could be found in the cerebellar grafts. Large neurons were also seen in the cortex grafts. Taken together, these data suggest that the athymic rat may serve as a useful tool for studies of central nervous system tissue from otherwise immunologically incompatible species.
将来自大脑皮层和小脑的人类胎儿组织碎片移植到成年无胸腺裸鼠的眼前房。这些移植物取自妊娠8至11周进行的选择性常规流产后回收的组织碎片。小脑和大脑皮层移植物在眼前房存活并生长了1至4个月。移植物从宿主虹膜逐渐缓慢地形成血管。在眼内放置4个月后，移植物形成了具有层粘连蛋白免疫反应性壁的血管，并且在神经毡中含有相对大量的胶质纤维酸性蛋白和神经丝免疫反应性物质。对移植物进行细胞外动作电位记录，发现了自发活动的神经元，其动作电位波形与在未成熟啮齿动物中观察到的相似。在形态学上，移植物没有排斥迹象。在小脑移植物中可以发现类似于浦肯野细胞的大神经元簇和带，以及较小的颗粒样细胞的密集聚集体。在皮层移植物中也可见到大神经元。综上所述，这些数据表明，无胸腺大鼠可能是研究来自其他免疫不相容物种的中枢神经系统组织的有用工具。
