Chen Xinjin, Fan Bolin, Fan Chenlong, Wang Zhongliang, Wangkahart Eakapol, Huang Yucong, Huang Yu, Jian Jichang, Wang Bei
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, PR China.
Research Unit of Excellence for Tropical Fisheries and Technology, Division of Fisheries, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Khamriang Sub-District, Kantarawichai, Mahasarakham, Thailand.
Proteome Sci. 2021 Nov 10;19(1):14. doi: 10.1186/s12953-021-00182-y.
Streptococcus agalactiae is a common colonizer of the rectovaginal tract and lead to infectious diseases of neonatal and non-pregnant adults, which also causes infectious disease in fish and a zoonotic risk as well. Lysine crotonylation (Kcr) is a kind of histone post-translational modifications discovered in 2011. In yeast and mammals, Kcr function as potential enhancers and promote gene expression. However, lysine crotonylation in S. agalactiae has not been studied yet.
In this study, the crotonylation profiling of fish pathogen, S. agalactiae was investigated by combining affinity enrichment with LC MS/MS. The Kcr modification of several selected proteins were further validated by Western blotting.
In the present study, we conducted the proteome-wide profiling of Kcr in S. agalactiae and identified 241 Kcr sites from 675 screened proteins for the first time. Bioinformatics analysis showed that 164 sequences were matched to a total of six definitively conserved motifs, and many of them were significantly enriched in metabolic processes, cellular process, and single-organism processes. Moreover, four crotonylation modified proteins were predicted as virulence factors or to being part of the quorum sensing system PTMs on bacteria. The data are available via ProteomeXchange with identifier PXD026445.
These data provide a promising starting point for further functional research of crotonylation in bacterial virulence in S. agalactiae.
无乳链球菌是直肠阴道道常见的定植菌，可导致新生儿和非妊娠成年人的感染性疾病，它还会引发鱼类的感染性疾病以及人畜共患病风险。赖氨酸巴豆酰化（Kcr）是2011年发现的一种组蛋白翻译后修饰。在酵母和哺乳动物中，Kcr作为潜在的增强子发挥作用并促进基因表达。然而，无乳链球菌中的赖氨酸巴豆酰化尚未得到研究。
在本研究中，通过亲和富集与液相色谱串联质谱联用，对鱼类病原体无乳链球菌的巴豆酰化图谱进行了研究。通过蛋白质免疫印迹进一步验证了几种选定蛋白质的Kcr修饰。
在本研究中，我们对无乳链球菌中的Kcr进行了全蛋白质组分析，首次从675种筛选出的蛋白质中鉴定出241个Kcr位点。生物信息学分析表明，164个序列与总共6个明确保守的基序相匹配，其中许多在代谢过程、细胞过程和单细胞过程中显著富集。此外，预测有4种巴豆酰化修饰的蛋白质为毒力因子或属于细菌群体感应系统的翻译后修饰的一部分。数据可通过ProteomeXchange获得，标识符为PXD026445。
这些数据为进一步研究无乳链球菌中巴豆酰化在细菌毒力中的功能提供了一个有前景的起点。
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