Aizawa Kenta, Takagi Hiroki, Kokubo Eri, Takada Masayasu
1 Research Institute, Nihon Shokuhin Kako Co., Ltd.
2 Wellness & Nutritional Science Institute, Morinaga Milk Industry Co., Ltd.
J Appl Glycosci (1999). 2021 Aug 22;68(3):53-61. doi: 10.5458/jag.jag.JAG-2021_0001. eCollection 2021.
Carbohydrate materials that produce lower postprandial blood glucose increase are required for diabetic patients. To develop slowly digestible carbohydrates, the effect of degree of polymerization (DP) of α-1,6 glucan on its digestibility was investigated and . We prepared four fractions of α-1,6 glucan composed primarily of DP 3-9, DP 10-30, DP 31-150, and DP 151+ by fractionating a dextran hydrolysate. An experiment using digestive enzymes showed that the glucose productions of DP 3-9, DP 10-30, DP 31-150, and DP 151+ were 70.3, 53.4, 28.2, and 19.2 % in 2 h, and 92.1, 83.9, 39.6, and 33.3 % in 24 h relative to dextrin, respectively. An glycemic response showed that the incremental area under the curve (iAUC) of blood glucose levels of α-1,6 glucan with DP 3-9, DP 10-30, DP 31-150, and DP 151+ were 99.5, 84.3, 65.4, and 40.1 % relative to dextrin, respectively. These results indicated that α-1,6 glucan with higher DP had stronger resistance to digestion and produced a smaller blood glucose response. DP 10-30 showed significantly lower maximum blood glucose levels than dextrin; however, no significant difference was observed in iAUC, indicating that DP 10-30 was slowly digestible. In addition, α-1,6 glucan was also produced using an enzymatic reaction with dextrin dextranase (DDase). This produced similar results to DP 10-30. The DDase product can be synthesized from dextrin at low cost. This glucan is expected to be useful as a slowly digestible carbohydrate source.
糖尿病患者需要能使餐后血糖升高幅度较小的碳水化合物。为了开发缓慢消化的碳水化合物，研究了α-1,6-葡聚糖的聚合度（DP）对其消化率的影响。我们通过对葡聚糖水解产物进行分级分离，制备了主要由DP 3-9、DP 10-30、DP 31-150和DP 151+组成的四个α-1,6-葡聚糖级分。一项使用消化酶的实验表明，相对于糊精，DP 3-9、DP 10-30、DP 31-150和DP 151+在2小时内的葡萄糖生成量分别为70.3%、53.4%、28.2%和19.2%，在24小时内分别为92.1%、83.9%、39.6%和33.3%。一项血糖反应表明，相对于糊精，DP 3-9、DP 10-30、DP 31-150和DP 151+的α-1,6-葡聚糖血糖水平的曲线下增量面积（iAUC）分别为99.5%、84.3%、65.4%和40.1%。这些结果表明，较高DP的α-1,6-葡聚糖对消化的抗性更强，产生的血糖反应更小。DP 10-30的最大血糖水平显著低于糊精；然而，在iAUC中未观察到显著差异，表明DP 10-30是缓慢消化的。此外，还使用糊精葡聚糖酶（DDase）通过酶促反应制备了α-1,6-葡聚糖。这产生了与DP 10-30相似的结果。DDase产物可以以低成本从糊精合成。这种葡聚糖有望作为一种缓慢消化的碳水化合物来源。
