Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat- Introgression Line M8664-3.

Stripe rust, caused by f. sp. (), is one of the most widespread and destructive fungal diseases of wheat worldwide. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust. Therefore, it is necessary to use new resistance genes to breed resistant wheat varieties. A single dominant gene temporarily designated as , from a wheat- introgression line M8664-3 highly resistant to Chinese predominant races, is a potentially valuable source of stripe rust resistance for breeding. Herein, based on previous chromosome location results (bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59) and expression change information of candidate genes and bioinformatics analysis, several candidate genes with significantly different expression changes were then selected and verified by virus-induced gene silencing (VIGS). Two of the candidate genes temporarily designated as [containing plastid lipid-associated proteins (PAP)_fibrillin domain in its protein] and [containing Pescadillo and breast cancer tumour suppressor protein C-terminus (BRCT) domain in its protein], produced the most significant resistance changes in the wheat- interaction system after silencing. These two genes were further verified by -mediated wheat genetic transformation technology. According to the identification of disease resistance, the resistance function of the candidate gene was further verified. Then, the expression of under hormone treatment indicated that may be related to the salicylic acid (SA) and abscisic acid (ABA) signaling pathways. Combined with the expression of in response to environmental stress stimulation, it can be reasonably speculated that plays an important role in the resistance of wheat to and is involved in abiotic stress pathways.