State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
Plant Dis. 2018 May;102(5):1001-1007. doi: 10.1094/PDIS-05-17-0671-RE. Epub 2018 Mar 22.
Wheat stripe rust is one of the most damaging diseases of wheat worldwide. The wheat-Leymus mollis introgression line M8664-3 exhibits all-stage resistance to Chinese stripe rust races. Genetic analysis of stripe rust resistance was performed by crossing M8664-3 with the susceptible line Mingxian169. Analysis of the disease resistance of F and F populations revealed that its resistance to Chinese stripe rust race 33 (CYR33) is controlled by a single dominant gene, temporarily designated as YrM8664-3. Genetic and physical mapping showed that YrM8664-3 is located in bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59 on chromosome 4AL and is flanked by single-nucleotide polymorphism markers AX111655681 and AX109496237 with genetic distances of 5.3 and 2.3 centimorgans, respectively. Resistance spectrum and position analyses indicated that YrM8664-3 may be a novel gene. Molecular detection using the markers linked to YrM8664-3 with wheat varieties commonly cultivated and wheat-L. mollis-derived lines showed that YrM8664-3 is also present in other wheat-L. mollis introgression lines but absent in commercial common wheat cultivars. Thus, YrM8664-3 is a potentially valuable source of stripe rust resistance for breeding.
小麦条锈病是全球范围内对小麦危害最大的病害之一。小麦-赖草渐渗系 M8664-3 对中国条锈菌生理小种表现出全生育期抗性。通过与感病系明选 169 杂交，对条锈病抗性进行了遗传分析。F1 和 F2 群体的抗病性分析表明，其对中国条锈菌生理小种 33（CYR33）的抗性由一个显性单基因控制，暂时命名为 YrM8664-3。遗传和物理图谱分析表明，YrM8664-3 位于 4AL13-0.59-0.66 -bin 内，靠近 4AL12-0.43-0.59 染色体，由单核苷酸多态性标记 AX111655681 和 AX109496237 侧翼，遗传距离分别为 5.3 和 2.3 厘摩。抗性谱和定位分析表明，YrM8664-3 可能是一个新基因。利用与 YrM8664-3 紧密连锁的标记对广泛种植的小麦品种和小麦-赖草衍生系进行分子检测表明，YrM8664-3 也存在于其他小麦-赖草渐渗系中，但不存在于商业普通小麦品种中。因此，YrM8664-3 是一种具有潜在价值的抗条锈病育种资源。
