Lozano Clément, Lebaron Philippe, Matallana-Surget Sabine
Division of Biological and Environmental Sciences, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom.
Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes, USR3579, Observatoire Océanologique, Sorbonne Université, Banyuls-sur-mer, France.
PeerJ. 2021 Nov 1;9:e12278. doi: 10.7717/peerj.12278. eCollection 2021.
UV filters are toxic to marine bacteria that dominate the marine biomass. Ecotoxicology often studies the organism response but rarely integrates the toxicity mechanisms at the molecular level. In this study, comparative genomics between UV filters sensitive and resistant bacteria were conducted in order to unravel the genes responsible for a resistance phenotype. The genomes of two environmentally relevant Bacteroidetes and three Firmicutes species were compared through pairwise comparison. Larger genomes were carried by bacteria exhibiting a resistant phenotype, favoring their ability to adapt to environmental stresses. While the antitoxin and CRISPR systems were the only distinctive features in resistant Bacteroidetes, Firmicutes displayed multiple unique genes that could support the difference between sensitive and resistant phenotypes. Several genes involved in ROS response, vitamin biosynthesis, xenobiotic degradation, multidrug resistance, and lipophilic compound permeability were shown to be exclusive to resistant species. Our investigation contributes to a better understanding of UV filters resistance phenotypes, by identifying pivotal genes involved in key pathways.
紫外线过滤器对占海洋生物量主导地位的海洋细菌有毒。生态毒理学通常研究生物体的反应，但很少在分子水平上整合毒性机制。在本研究中，对紫外线过滤器敏感菌和耐药菌进行了比较基因组学研究，以揭示导致耐药表型的基因。通过两两比较，对两种与环境相关的拟杆菌属和三种厚壁菌门物种的基因组进行了比较。表现出耐药表型的细菌携带更大的基因组，这有利于它们适应环境压力的能力。虽然抗毒素和CRISPR系统是耐药拟杆菌属中唯一的独特特征，但厚壁菌门显示出多个独特基因，这些基因可能支持敏感表型和耐药表型之间的差异。一些参与活性氧反应、维生素生物合成、异源生物降解、多药耐药性和亲脂性化合物通透性的基因被证明是耐药物种所特有的。我们的研究通过识别关键途径中涉及的关键基因，有助于更好地理解紫外线过滤器耐药表型。
