癌症衍生免疫球蛋白G的表达上调与胶质瘤进展相关。
Upregulated Expression of Cancer-Derived Immunoglobulin G Is Associated With Progression in Glioma.
作者信息
Wang Guohui, Li Haonan, Pan Jie, Yan Tianfang, Zhou Huandi, Han Xuetao, Su Linlin, Hou Liubing, Xue Xiaoying
机构信息
Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Department of Central Laboratory, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
出版信息
Front Oncol. 2021 Oct 25;11:758856. doi: 10.3389/fonc.2021.758856. eCollection 2021.
OBJECTIVE
Gliomas are the most aggressive intracranial tumors accounting for the vast majority of brain tumors with very poor prognosis and overall survival (OS). Cancer-derived immunoglobulin G (cancer-IgG) has been found to be widely expressed in several malignancies such as breast cancer, colorectal cancer, and lung cancer. Cancer-IgG could promote tumorigenesis and progression. However, its role in glioma has not been revealed yet.
METHODS
We mined open databases including the Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and the Gene Expression Omnibus (GEO) to study the role of , which encodes cancer-IgG in glioma. Examination of the differential expression of was carried out in the GEO and TCGA databases. Furthermore, its expression in different molecular subtypes was analyzed. Stratified analysis was performed with clinical features. Subsequently, immune infiltration analysis was conducted using single-sample gene set enrichment analysis (ssGSEA). GSEA was performed to reveal the mechanisms of . Lastly, immunohistochemistry was processed to validate our findings.
RESULTS
In this study, we found that the expression of was higher in glioma and molecular subtypes with poor prognosis. The overall survival of patients with a high expression of was worse in the stratified analysis. Immune infiltration analysis indicated that the expression level of was positively correlated with the stromal score, ESTIMATE score, and immune score and negatively correlated with tumor purity. Results from the GSEA and DAVID demonstrated that may function in phagosome, antigen processing and presentation, extracellular matrix structural constituent, antigen binding, and collagen-containing extracellular matrix. Finally, immunohistochemistry assay validated our findings that patients with a high expression of cancer-IgG had poor OS and disease-free survival (DFS).
CONCLUSION
Cancer-IgG is a promising biomarker of diagnosis and treatment for patients with glioma.
目的
胶质瘤是最具侵袭性的颅内肿瘤，占脑肿瘤的绝大多数，预后和总生存期（OS）很差。癌源性免疫球蛋白G（癌症-IgG）已被发现在多种恶性肿瘤中广泛表达，如乳腺癌、结直肠癌和肺癌。癌症-IgG可促进肿瘤发生和进展。然而，其在胶质瘤中的作用尚未揭示。
方法
我们挖掘了包括中国胶质瘤基因组图谱（CGGA）、癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）在内的公开数据库，以研究编码癌症-IgG的 在胶质瘤中的作用。在GEO和TCGA数据库中对 的差异表达进行检测。此外，分析了其在不同分子亚型中的表达。根据临床特征进行分层分析。随后，使用单样本基因集富集分析（ssGSEA）进行免疫浸润分析。进行基因集富集分析（GSEA）以揭示 的作用机制。最后，进行免疫组织化学以验证我们的发现。
结果
在本研究中，我们发现 在胶质瘤及预后较差的分子亚型中表达较高。在分层分析中， 高表达患者的总生存期较差。免疫浸润分析表明， 的表达水平与基质评分、ESTIMATE评分和免疫评分呈正相关，与肿瘤纯度呈负相关。GSEA和DAVID的结果表明， 可能在吞噬体、抗原加工和呈递、细胞外基质结构成分、抗原结合和含胶原细胞外基质中发挥作用。最后，免疫组织化学检测验证了我们的发现，即癌症-IgG高表达的患者总生存期和无病生存期（DFS）较差。
结论
癌症-IgG是胶质瘤患者诊断和治疗的一个有前景的生物标志物。
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