Kudira Ramesh, Sharma Bal Krishan, Mullen Mary, Mohanty Sujit K, Donnelly Bryan, Tiao Gregory M, Miethke Alexander
Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Hematology Department. Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Bio Protoc. 2021 Oct 20;11(20):e4192. doi: 10.21769/BioProtoc.4192.
Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate in response to endogenous or exogenous signals leading to cholangiopathies, inflammation, fibrosis, and cholangiocarcinoma. Cholangiocytes exhibit anatomical and functional heterogeneity, and understanding such diversified functions will potentially help in finding effective therapies for various cholestatic liver diseases. To perform such functional studies, effective cholangiocyte isolation and culture procedures are needed. This protocol will aid in easy isolation and expansion of cholangiocytes from the liver.
胆管细胞是肝内和肝外胆管内衬的上皮细胞。胆管细胞在肝脏中发挥关键的生理功能。肝细胞合成的胆汁分泌到胆小管中，进一步储存在胆囊中，最终排入十二指肠。由于肝损伤，胆管上皮会对内源性或外源性信号作出增殖反应，导致胆管病、炎症、纤维化和胆管癌。胆管细胞表现出解剖学和功能上的异质性，了解这些多样化的功能可能有助于找到针对各种胆汁淤积性肝病的有效治疗方法。为了进行此类功能研究，需要有效的胆管细胞分离和培养程序。本方案将有助于从肝脏中轻松分离和扩增胆管细胞。
