School of Medicine and Surgery, Australian National University, Canberra, Australian Capital Territory, Australia.
Institute for Health Research, University of Notre Dame Australia, Fremantle, Western Australia, Australia.
J Obstet Gynaecol Res. 2022 Jan;48(1):119-128. doi: 10.1111/jog.15086. Epub 2021 Nov 10.
To examine whether there are differences in the vaginal microbiome of women who miscarry compared to those who have normal pregnancy outcomes.
Prospective observational study conducted at the Canberra Hospital, Australia, with 24 participant women in the first trimester of pregnancy. The vaginal microbiomes of the 24 women were characterized using sequencing analysis of the V4 region of the 16S rRNA gene employing an Illumina MiSeq instrument with QIAGEN reagents. Vaginal microbiome data were correlated with pregnancy clinical metadata.
Ordination plots showed differences in the composition of microbiomes of women who miscarried and controls. In nulliparous women, Lactobacillus crispatus was the dominant bacterium in 50% of women. Lactobacillus iners was the dominant bacterium in 50% of women with a history of prior miscarriage and a miscarriage in the study compared to 15% (p = 0.011) in those with no history of miscarriage and no miscarriage in the study. There were significant differences in the number of operational taxonomic units and the richness of the microbiomes of women who miscarried compared to those who delivered at term. Eight taxa were found in different relative abundances in both groups of women.
The study indicated that the composition of the vaginal microbiome varies with pregnancy history. Also, there was a significant difference in the vaginal microbiomes between women who suffered miscarriage and those who continued to term delivery both in the overall microbiome populations and in the abundances of individual taxa.
研究自然流产和正常妊娠孕妇的阴道微生物组是否存在差异。
本研究为在澳大利亚堪培拉医院进行的前瞻性观察研究，纳入 24 名处于妊娠早期的孕妇。采用 Illumina MiSeq 仪器和 QIAGEN 试剂，对 16S rRNA 基因 V4 区进行测序分析，以对 24 名孕妇的阴道微生物组进行特征描述。将阴道微生物组数据与妊娠临床元数据相关联。
排序图显示，自然流产和对照组孕妇的微生物组组成存在差异。在未产妇中，50%的女性阴道中优势菌为乳酸杆菌 crispatus。与无流产史和研究中无流产的女性相比（p=0.011），有既往流产史和研究中流产的女性中，优势菌为乳酸杆菌 iners 的占 50%。与足月分娩的女性相比，自然流产的女性阴道微生物组的操作分类单元数量和丰富度存在显著差异。两组女性的 8 种细菌的相对丰度存在差异。
该研究表明，阴道微生物组的组成随妊娠史而变化。此外，在自然流产和继续足月分娩的女性中，无论是在整体微生物组人群中，还是在个体分类群的丰度方面，阴道微生物组均存在显著差异。
